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Literature summary for 7.3.2.7 extracted from
- Caenorhabditis elegans expresses a functional ArsA (2007), FEBS J., 274, 2566-2572.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of the enzyme as maltose-binding-protein fusion protein in Escherichia coli | Caenorhabditis elegans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | asna-1-mutant nematodes are more sensitive to As(III) and Sb(III) toxicity than are wild-type animals | Caenorhabditis elegans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00004 | - |
Sb3+ | - |
Caenorhabditis elegans |  |
| 0.021 | - |
ATP | pH 7.4, 37°C, wild-type enzyme in absence of Sb3+ | Caenorhabditis elegans | |
| 0.026 | - |
ATP | pH 7.4, 37°C, wild-type enzyme in presence of Sb3+ | Caenorhabditis elegans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| As3+ | activates the ATPase activity of the ASNA-1 protein 2.0fold, Km is 3.54 mM at ATP saturation | Caenorhabditis elegans | |
| Mg2+ | required | Caenorhabditis elegans | |
| Sb3+ | activates the ATPase activity of the ASNA-1 protein 4.6fold, Km is 0.04 mM at ATP saturation | Caenorhabditis elegans | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + arsenite/in | Caenorhabditis elegans | ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview | ADP + phosphate + arsenite/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | P30632 | gene asna-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant maltose-binding-protein fusion protein from Escherichia coli by amylose affinity chromatography | Caenorhabditis elegans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28.87 | - |
purified recombinant MBP-fusion enzyme stimulated by 0.5 mM Sb3+ | Caenorhabditis elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + arsenite/in | - |
Caenorhabditis elegans | ADP + phosphate + arsenite/out | - |
? | |
| ATP + H2O + arsenite/in | ASNA-1 , encoding a functional ArsA ATPase, is critical for As(III) and Sb(III) tolerance in the intact organism, the enzyme does not provide resistance to other metals, e.g. Cu2+ or Pb2+, overview | Caenorhabditis elegans | ADP + phosphate + arsenite/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ArsA ATPase | - |
Caenorhabditis elegans |
| ASNA-1 | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Caenorhabditis elegans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 37 | - |
Caenorhabditis elegans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Caenorhabditis elegans |
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Release 2023.1 (January 2023)
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