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Literature summary for 7.3.2.6 extracted from

  • Taveirne, M.E.; Sikes, M.L.; Olson, J.W.
    Molybdenum and tungsten in Campylobacter jejuni: their physiological role and identification of separate transporters regulated by a single ModE-like protein (2009), Mol. Microbiol., 74, 758-771.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
tupA and tupB are two genes in the tungsten transport locus which are divergently transcribed, genetic regulation of tupA/tupB and modA, overview Campylobacter jejuni

Protein Variants

Protein Variants Comment Organism
additional information construction of tupB and modA/tupB defective mutants. Supplementation with Na2MoO4 decreases formate dehydrogenase activity in tmutant upB:KAN to 37% of wild-type level, suggesting a preference of tungsten over molybdenum by Tup. When both transport systems are disrupted, formate dehydrogenase activity falls to 5% of the wild-type level, but activity is restored to near wild-type levels only with addition of 1 mM Na2WO4, supplementation with 1 mM Na2MoO4 only restores 20% of formate dehydrogenase activity Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + tungstate/out Campylobacter jejuni
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ADP + phosphate + tungstate/in
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?

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
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gene tupA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + tungstate/out
-
Campylobacter jejuni ADP + phosphate + tungstate/in
-
?
additional information mod , EC 3.6.3.29, is primarily a molybdenum transporter that can also transport tungsten, while tup is a tungsten-specific transporter Campylobacter jejuni ?
-
?

Synonyms

Synonyms Comment Organism
tungstate transporter
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Campylobacter jejuni
tungsten-specific transporter
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Campylobacter jejuni
TupA
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Campylobacter jejuni

Expression

Organism Comment Expression
Campylobacter jejuni genetic regulation of tupA and modA, overview additional information

General Information

General Information Comment Organism
metabolism ModE-like protein regulates both transporters, repressing mod, EC 3.6.3.29, in the presence of both molybdenum and tungsten and tup only in the presence of tungsten. Like other ModE proteins, the Campylobacter jejuni ModE binds DNA through a helix-turn-helix DNA binding domain, but unlike other members of the ModE family it does not have a metal binding domain Campylobacter jejuni
physiological function the enzyme provides tungsten for incorporation into the format dehydrogenase, formate dehydrogenase is active in the presence of either tungsten or molybdenum Campylobacter jejuni