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Literature summary for 7.3.2.6 extracted from

  • Bevers, L.E.; Hagedoorn, P.; Krijger, G.C.; Hagen, W.R.
    Tungsten transport protein A (WtpA) in Pyrococcus furiosus: the first member of a new class of tungstate and molybdate transporters (2006), J. Bacteriol., 188, 6498-6505.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.3.2.6

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the extremely high affinity of the enzyme for tungstate results in a very steep binding curve Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 native PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + tungstate/out Pyrococcus furiosus the very high affinity WtpABC transporter explains the ability of Pyrococcus furiosus cells to scavenge traces of tungstate from growth medium ADP + phosphate + tungstate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U4K5 tungstate transport protein A, part of the ABC transporter system
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + tungstate/out the very high affinity WtpABC transporter explains the ability of Pyrococcus furiosus cells to scavenge traces of tungstate from growth medium Pyrococcus furiosus ADP + phosphate + tungstate/in
-
 ?
ATP + H2O + tungstate/out although reversible binding of molybdate or tungstate by the soluble component WtpA of the archaeal WtpABC transporter enforces a quasi-octahedral coordination onto the molybdenum or tungsten, this does not induce any redox chemistry of the +VI state Pyrococcus furiosus ADP + phosphate + tungstate/in
-
 ?
ATP + H2O + tungstate/out when the protein is incubated with a mixture of tungstate and molybdate, the amount of bound tungstate is significantly higher, even in the presence of an excess of molybdate during the incubation. WtpA is able to bind tungstate and molybdate, with a preference for tungstate, without changing the oligomeric state of the protein Pyrococcus furiosus ADP + phosphate + tungstate/in
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
PF0080
-
 Pyrococcus furiosus
tungstate-specific ABC transporter
-
 Pyrococcus furiosus
WptA tungstate transport protein A, part of the ABC transporter system Pyrococcus furiosus
WtpABC
-
 Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 6 h, no precipiotation is observed Pyrococcus furiosus