KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | oxoanion binding kinetic of ModA, overview | Oleidesulfovibrio alaskensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Oleidesulfovibrio alaskensis | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, recombinant enzyme, SDS-PAGE | Oleidesulfovibrio alaskensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + molybdate/out | Oleidesulfovibrio alaskensis | - |
ADP + phosphate + molybdate/in | - |
? | |
ATP + H2O + molybdate/out | Oleidesulfovibrio alaskensis G20 | - |
ADP + phosphate + molybdate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oleidesulfovibrio alaskensis | - |
genes modABC, organized in the ModABC operon | - |
Oleidesulfovibrio alaskensis G20 | - |
genes modABC, organized in the ModABC operon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + molybdate/out | - |
Oleidesulfovibrio alaskensis | ADP + phosphate + molybdate/in | - |
? | |
ATP + H2O + molybdate/out | - |
Oleidesulfovibrio alaskensis G20 | ADP + phosphate + molybdate/in | - |
? | |
additional information | component TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate, quantitative analysis of metal binding by isothermal titration calorimetry, overview | Oleidesulfovibrio alaskensis | ? | - |
? | |
additional information | component TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate, quantitative analysis of metal binding by isothermal titration calorimetry, overview | Oleidesulfovibrio alaskensis G20 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, recombinant enzyme, SDS-PAGE | Oleidesulfovibrio alaskensis |
Synonyms | Comment | Organism |
---|---|---|
ModABC | - |
Oleidesulfovibrio alaskensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oleidesulfovibrio alaskensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Oleidesulfovibrio alaskensis |
Organism | Comment | Expression |
---|---|---|
Oleidesulfovibrio alaskensis | the periplasmic protein involved in molybdate transport, ModA, is downregulated under high molybdate concentration stress conditions | down |
General Information | Comment | Organism |
---|---|---|
physiological function | the ModABC system is involved in the cellular uptake of molybdate and belongs to the ABC (ATP binding cassette)-type transporter systems. The ModA component is a periplasmic protein that binds molybdate anions, which are then transported through the membrane by the ModB component using ATP hydrolysis as the energy source, the reaction is catalyzed by the ModC component. The genes encoding the three components are organized in an operon modABC regulated by a transcription factor known as ModE. Under an excess of molybdate, ModE binds molybdate ions, suffers conformational changes and dimerizes. This metal-protein complex binds to a specific DNA sequence (located upstream of the modABC operon) and downregulates the expression of proteins involved in molybdenum uptake. Under oxoanion starvation, the component A binds molybdate and interacts with the component B to actively transport molybdate or tungstate from the periplasm to the cytoplasm. The ModABC transport system and, more specifically, the component A may constitute the first selection gate from which cells differentiate between Mo and W. Regulation of the transport enzyme complex, overview | Oleidesulfovibrio alaskensis |