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Literature summary for 7.3.2.5 extracted from

  • Otrelo-Cardoso, A.R.; Nair, R.R.; Correia, M.A.; Rivas, M.G.; Santos-Silva, T.
    TupA: a tungstate binding protein in the periplasm of Desulfovibrio alaskensis G20 (2014), Int. J. Mol. Sci., 15, 11783-11798.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information oxoanion binding kinetic of ModA, overview Oleidesulfovibrio alaskensis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Oleidesulfovibrio alaskensis 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
x * 29000, recombinant enzyme, SDS-PAGE Oleidesulfovibrio alaskensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + molybdate/out Oleidesulfovibrio alaskensis
-
ADP + phosphate + molybdate/in
-
?
ATP + H2O + molybdate/out Oleidesulfovibrio alaskensis G20
-
ADP + phosphate + molybdate/in
-
?

Organism

Organism UniProt Comment Textmining
Oleidesulfovibrio alaskensis
-
genes modABC, organized in the ModABC operon
-
Oleidesulfovibrio alaskensis G20
-
genes modABC, organized in the ModABC operon
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + molybdate/out
-
Oleidesulfovibrio alaskensis ADP + phosphate + molybdate/in
-
?
ATP + H2O + molybdate/out
-
Oleidesulfovibrio alaskensis G20 ADP + phosphate + molybdate/in
-
?
additional information component TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate, quantitative analysis of metal binding by isothermal titration calorimetry, overview Oleidesulfovibrio alaskensis ?
-
?
additional information component TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate, quantitative analysis of metal binding by isothermal titration calorimetry, overview Oleidesulfovibrio alaskensis G20 ?
-
?

Subunits

Subunits Comment Organism
? x * 29000, recombinant enzyme, SDS-PAGE Oleidesulfovibrio alaskensis

Synonyms

Synonyms Comment Organism
ModABC
-
Oleidesulfovibrio alaskensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oleidesulfovibrio alaskensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Oleidesulfovibrio alaskensis

Expression

Organism Comment Expression
Oleidesulfovibrio alaskensis the periplasmic protein involved in molybdate transport, ModA, is downregulated under high molybdate concentration stress conditions down

General Information

General Information Comment Organism
physiological function the ModABC system is involved in the cellular uptake of molybdate and belongs to the ABC (ATP binding cassette)-type transporter systems. The ModA component is a periplasmic protein that binds molybdate anions, which are then transported through the membrane by the ModB component using ATP hydrolysis as the energy source, the reaction is catalyzed by the ModC component. The genes encoding the three components are organized in an operon modABC regulated by a transcription factor known as ModE. Under an excess of molybdate, ModE binds molybdate ions, suffers conformational changes and dimerizes. This metal-protein complex binds to a specific DNA sequence (located upstream of the modABC operon) and downregulates the expression of proteins involved in molybdenum uptake. Under oxoanion starvation, the component A binds molybdate and interacts with the component B to actively transport molybdate or tungstate from the periplasm to the cytoplasm. The ModABC transport system and, more specifically, the component A may constitute the first selection gate from which cells differentiate between Mo and W. Regulation of the transport enzyme complex, overview Oleidesulfovibrio alaskensis