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Literature summary for 7.3.2.5 extracted from

  • Makdessi, K.; Fritsche, K.; Pich, A.; Andreesen, J.R.
    Identification and characterization of the cytoplasmic tungstate/molybdate-binding protein (Mop) from Eubacterium acidaminophilum (2004), Arch. Microbiol., 181, 45-51.
    View publication on PubMed
Search for more literature for 7.3.2.5

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed in Escherichia coli XL 1-Blue as a C-terminal Strep-tag fusion protein Peptoclostridium acidaminophilum

Protein Variants

Protein Variants Comment Organism
additional information substituting either Lys, Leu or Glu for Arg-6 using site-directed mutagenesis, positive charge of ARg-6, located in the conserved SARN region of Mop, is not directly involved in oxyanion binding Peptoclostridium acidaminophilum

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
8100
-
 6 * 8100, SDS-PAGE, recombinant enzyme Peptoclostridium acidaminophilum
48000
-
 gel filtration, native enzyme Peptoclostridium acidaminophilum

Organism

Organism UniProt Comment Textmining
Peptoclostridium acidaminophilum
-
 strain DSM3953
-
Peptoclostridium acidaminophilum DSM 3953
-
 strain DSM3953
-

Purification (Commentary)

Purification (Comment) Organism
by affinity chromatography Peptoclostridium acidaminophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + chromate/out
-
 Peptoclostridium acidaminophilum ?
-
 ?
ATP + H2O + chromate/out
-
 Peptoclostridium acidaminophilum DSM 3953 ?
-
 ?
ATP + H2O + molybdate/out
-
 Peptoclostridium acidaminophilum ?
-
 ?
ATP + H2O + molybdate/out
-
 Peptoclostridium acidaminophilum DSM 3953 ?
-
 ?
ATP + H2O + tungstate/out
-
 Peptoclostridium acidaminophilum ?
-
 ?
ATP + H2O + tungstate/out
-
 Peptoclostridium acidaminophilum DSM 3953 ?
-
 ?
additional information sulfate and phosphate have no effect on Mop mobility Peptoclostridium acidaminophilum ?
-
 ?
additional information sulfate and phosphate have no effect on Mop mobility Peptoclostridium acidaminophilum DSM 3953 ?
-
 ?

Subunits

Subunits Comment Organism
homohexamer 6 * 8100, SDS-PAGE, recombinant enzyme Peptoclostridium acidaminophilum

Synonyms

Synonyms Comment Organism
MOP
-
 Peptoclostridium acidaminophilum
tungstate/molybdate-binding protein
-
 Peptoclostridium acidaminophilum