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Literature summary for 7.3.2.4 extracted from
- Molecular basis of nitrate uptake by the plant nitrate transporter NRT1.1 (2014), Nature, 507, 68-72.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Saccharomyces cerevisiae | Arabidopsis thaliana |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| apo and nitrate-bound enzyme, hanging drop vapor diffusion method, using 22% (w/v) PEG 400, 0.05 M sodium citrate pH 4.5, 0.07 M sodium chloride and 1.5% (w/v) PEG 600 | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H356A | the mutation results in complete loss of nitrate binding ability | Arabidopsis thaliana |
| T101D | the variant shows increased nitrate uptake of approximately 2.8fold compared to the wild type enzyme | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + nitrate/out | Arabidopsis thaliana | - |
ADP + phosphate + nitrate/in | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q05085 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + nitrate/out | - |
Arabidopsis thaliana | ADP + phosphate + nitrate/in | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrate transporter | - |
Arabidopsis thaliana |
| NPF6.3 | - |
Arabidopsis thaliana |
| NRT1.1 | - |
Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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