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Literature summary for 7.3.2.4 extracted from

  • Yong, Z.; Kotur, Z.; Glass, A.D.
    Characterization of an intact two-component high-affinity nitrate transporter from Arabidopsis roots (2010), Plant J., 63, 739-748.
    View publication on PubMed
Search for more literature for 7.3.2.4

Protein Variants

Protein Variants Comment Organism
additional information generation of several mutants devoid of NAR2.1, e.g. Atnar2.1-35S:NAR2.1-myc mutant, complementation by expression of wild-type NAR2.1, phenotypes, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum membrane AtNAR2.1 Arabidopsis thaliana 5789
-
Golgi membrane AtNAR2.1 Arabidopsis thaliana 139
-
microsome AtNAR2.1 and AtNRT2.1 Arabidopsis thaliana
-
-
plasma membrane AtNAR2.1 and AtNRT2.1 Arabidopsis thaliana 5886
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 48000, AtNRT2.1, SDS-PAGE, + 2 * 26000, myc-tagged AtNAR2.1, SDS-PAGE, the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1 Arabidopsis thaliana
48000
-
 2 * 48000, AtNRT2.1, SDS-PAGE, + 2 * 26000, myc-tagged AtNAR2.1, SDS-PAGE, the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1 Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root cortical and epidermal cells Arabidopsis thaliana
-

Subunits

Subunits Comment Organism
tetramer 2 * 48000, AtNRT2.1, SDS-PAGE, + 2 * 26000, myc-tagged AtNAR2.1, SDS-PAGE, the functional unit for high-affinity nitrate influx may be a tetramer consisting of two subunits each of AtNRT2.1 and AtNAR2.1 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtNRT2.1
-
 Arabidopsis thaliana
nitrate transporter
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction mutants lacking AtNAR2.1 have virtually no high-affinity nitrate transport system capacity and exhibit extremely poor growth on low nitrate as the sole source of nitrogen. Near-normal growth and nitrate transport in the mutant are restored by transformation with myc-tagged AtNAR2.1 cDNA Arabidopsis thaliana
additional information AtNRT2.1 is a polypeptide of the Arabidopsis thaliana two-component inducible high-affinity nitrate transport system, IHATS, formed by AtNRT2.1 and AtNAR2.1, i.e AtNRT3.1.The monomeric form of AtNRT2.1 is the most abundant form, but the complex, rather than monomeric AtNRT2.1, is the form that is active in IHATS nitrate transport Arabidopsis thaliana