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Literature summary for 7.3.2.4 extracted from

  • Chopin, F.; Wirth, J.; Dorbe, M.F.; Lejay, L.; Krapp, A.; Gojon, A.; Daniel-Vedele, F.
    The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane (2007), Plant Physiol. Biochem., 45, 630-635.
    View publication on PubMed
Search for more literature for 7.3.2.4

Cloned(Commentary)

Cloned (Comment) Organism
a fusion protein between a 2-kb fragment of the ATNRT2.7 promoter and either the beta-glucuronidase or green fluorescent protein reporter gene stably introduced into the Arabidopsis thaliana ecotype Wassilewskija Arabidopsis thaliana
expressed as GFP-fusion protein using Agrobacterium tumefaciens, correct localization Arabidopsis thaliana
Xenopus laevis oocytes are injected with nuclease-free water or ATNRT2.7 mRNA, ATNRT2.7 mRNA-injected oocytes take up significantly more nitrate than water-injected controls Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane predominant localization Arabidopsis thaliana 16020
-
microsome
-
 Arabidopsis thaliana
-
-
vacuole vacuole membrane Arabidopsis thaliana 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, diffuse signal, SDS-PAGE Arabidopsis thaliana
57000
-
 x * 57000, calculated from the deduced amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana nitrate transport through the cell membrane ?
-
 ?
additional information Arabidopsis thaliana nitrate transport through the cell membrane, important for nitrate accumulation in seeds, influences the kinetics of seed germination, not involved in the nitrate uptake in roots or in nitrate distribution within the vegetative organs ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
 ecotype Wassilewskija
-
Arabidopsis thaliana
-
 ecotype Col-8
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-
root low level Arabidopsis thaliana
-
seed highly expressed in dry seeds, especially at the end of seed maturation, weak expression in the surrounding endosperm Arabidopsis thaliana
-
shoot higher enzyme level than in roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nitrate transport through the cell membrane Arabidopsis thaliana ?
-
 ?
additional information nitrate transport through the cell membrane, important for nitrate accumulation in seeds, influences the kinetics of seed germination, not involved in the nitrate uptake in roots or in nitrate distribution within the vegetative organs Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, diffuse signal, SDS-PAGE Arabidopsis thaliana
? x * 57000, calculated from the deduced amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtNRT2.1 high affinity nitrate transporter Arabidopsis thaliana
ATNRT2.7
-
 Arabidopsis thaliana