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Literature summary for 7.3.2.4 extracted from

  • Segonzac, C.; Boyer, J.C.; Ipotesi, E.; Szponarski, W.; Tillard, P.; Touraine, B.; Sommerer, N.; Rossignol, M.; Gibrat, R.
    Nitrate efflux at the root plasma membrane: identification of an Arabidopsis excretion transporter (2007), Plant Cell, 19, 3760-3777.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.3.2.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed as GUS-fusion protein in Arabidopsis thaliana Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information construction of a knockout mutant, no effect on plant growth, no changes in nitrate uptake upon acid load or medium acidification Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane accumulation of enzyme upon acid load or medium acidification Arabidopsis thaliana 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61099
-
 x * 61099, calculated from the deduced amino acid sequence Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana efflux nitrate transport through the cell membrane, increased transport rates in acidic conditions without enhancement of transcript levels but increased enzyme level resulting from posttranscriptional processes, involved in nitrate distribution although this seems not be essential for correct plant growth in standard growth conditions ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9M1E2 formerly unidentified protein; light and N-status does not affect enzyme level
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + nitrate-[nitrate-binding protein][side 1] = ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1] efflux transporter coupled to the ATP-dependent H+ pumping activity, transports intracellular nitrate through the membrane out of the cell Arabidopsis thaliana
a

Source Tissue

Source Tissue Comment Organism Textmining
root mainly in the cortex of mature roots Arabidopsis thaliana
-
shoot lower level than in roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information efflux nitrate transport through the cell membrane, increased transport rates in acidic conditions without enhancement of transcript levels but increased enzyme level resulting from posttranscriptional processes, involved in nitrate distribution although this seems not be essential for correct plant growth in standard growth conditions Arabidopsis thaliana ?
-
 ?

Subunits

Subunits Comment Organism
? x * 61099, calculated from the deduced amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
NAX1 nitrate excretion transporter, belongs to the NRT1/PTR transporter family Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Arabidopsis thaliana