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Literature summary for 7.3.2.4 extracted from

  • Navarro, F.J.; Machin, F.; Martin, Y.; Siverio, J.M.
    Down-regulation of eukaryotic nitrate transporter by nitrogen-dependent ubiquitinylation (2006), J. Biol. Chem., 281, 13268-13274.
    View publication on PubMed
Search for more literature for 7.3.2.4

Cloned(Commentary)

Cloned (Comment) Organism
mutants and GFP-fusion protein, GFP-fusion protein is active and correctly localized Ogataea angusta

Protein Variants

Protein Variants Comment Organism
DELTA232-286 enzyme does not disappear in response to glutamine Ogataea angusta
K243R no effect on down-regulation by glutamine Ogataea angusta
K243R/K253R reduced down-regulation in response to glutamine Ogataea angusta
K243R/K253R/K270R no ubiquitinylation, strongly reduced down-regulation in response to glutamine Ogataea angusta
K253R reduced down-regulation in response to glutamine Ogataea angusta
K253R/K270R reduced down-regulation in response to glutamine Ogataea angusta
K270R reduced down-regulation in response to glutamine Ogataea angusta

Inhibitors

Inhibitors Comment Organism Structure
additional information is degraded in the vacuole in response to glutamine, induced by nitrate and repressed by the preferred nitrogen sources ammonium or glutamine Ogataea angusta

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Ogataea angusta 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ogataea angusta nitrate and nitrite transport through the cell membrane, essential for nitrogen supply ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ogataea angusta
-
 different strains which are derivatives of the NCYC495 leu2 ura3 strain, enzyme is highly expressed in media containing nitrate as sole nitrogen source, addition of glutamine or ammonium decreased nitrate uptake and enzyme level
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information nitrogen sources trigger Ynt1 ubiquitinylation, Ynt1 becomes modified with ubiquitin during down-regulation as a result of nitrate assimilation or the shift to a preferred nitrogen source Ogataea angusta
phosphoprotein phosphorylation sites at the central cytosolic domain of the enzyme, not involved in the down-regulation of the enzyme Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nitrate and nitrite transport through the cell membrane, essential for nitrogen supply Ogataea angusta ?
-
 ?

Synonyms

Synonyms Comment Organism
Ynt1 high affinity bispecific nitrate/nitrite transporter Ogataea angusta
YNT1 gene product
-
 Ogataea angusta