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Literature summary for 7.3.2.4 extracted from

  • Sharma, V.; Noriega, C.E.; Rowe, J.J.
    Involvement of NarK1 and NarK2 proteins in transport of nitrate and nitrite in the denitrifying bacterium Pseudomonas aeruginosa PAO1 (2006), Appl. Environ. Microbiol., 72, 3802.
No PubMed abstract available
Search for more literature for 7.3.2.4

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pseudomonas aeruginosa
narK gene expressed in NarK2 and NarK1/NarK2 deletion mutants of Pseudomonas aeruginosa PAO1, narK gene expression is capable of restoring the anaerobic growth of Pseudomonas aeruginosa PAO1, although at reduced rates compared with the wild type Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants, NarK1 mutant grows almost normally, while NarK2 mutant shows strongly reduced generation times under nitrate-dependent anaerobic growth conditions, almost no growth of the NarK1/NarK2 double mutant, impaired growth can be restored by expression of the narK gene from Escherichia coli K-12 Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pseudomonas aeruginosa 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47300
-
 x * 47300, NarK1, calculated from the deduced amino acid sequence Pseudomonas aeruginosa
50600
-
 x * 50600, NarK2, calculated from the deduced amino acid sequence Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Pseudomonas aeruginosa important for nitrate/nitrite transport through the cell membrane, NarK2 protein is required as a nitrate/nitrite transporter under denitrifying conditions. ?
-
 ?
additional information Escherichia coli nitrate transport through the cell membrane ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Pseudomonas aeruginosa
-
 wild type and deletion mutants, NarK1 deletion does not affect nitrate uptake while NarK2 deletion causes strongly reduced nitrate uptake rates
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information important for nitrate/nitrite transport through the cell membrane, NarK2 protein is required as a nitrate/nitrite transporter under denitrifying conditions. Pseudomonas aeruginosa ?
-
 ?
additional information nitrate transport through the cell membrane Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
? x * 47300, NarK1, calculated from the deduced amino acid sequence Pseudomonas aeruginosa
? x * 50600, NarK2, calculated from the deduced amino acid sequence Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
narK gene product
-
 Escherichia coli
NarK1
-
 Pseudomonas aeruginosa
NarK2
-
 Pseudomonas aeruginosa