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Literature summary for 7.3.2.4 extracted from
- The nitrate/nitrite ABC transporter of Phormidium laminosum: phosphorylation state of NrtA is not involved in its substrate binding activity (2006), Biochim. Biophys. Acta, 1760, 172-181.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain M15 | Phormidium laminosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ammonium | - |
Phormidium laminosum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasmic membrane | - |
Phormidium laminosum | - |
- |
| periplasm | - |
Phormidium laminosum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
truncated NrtA, gel filtration | Phormidium laminosum |
| 38770 | - |
calculated from deduced amino acid sequence | Phormidium laminosum |
| 38890 | - |
His-tagged NrtA1, MALDI-TOF | Phormidium laminosum |
| 45000 | - |
His-tagged NrtA1, silver staining and immunoblotting of two-dimensional gel electrophoresis | Phormidium laminosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phormidium laminosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni2+-chelating Sepharose fast-flow column chromatography | Phormidium laminosum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + H2O + nitrate-[nitrate-binding protein][side 1] = ADP + phosphate + nitrate[side 2] + [nitrate-binding protein][side 1] | phosphorylated His-tagged NrtA1 specifically binds both nitrite and nitrate with high affinity and appears to be saturated at anion concentrations higher than 0.04 mM at pH 8.0 and 45°C | Phormidium laminosum |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, gel filtration | Phormidium laminosum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| nitrate/nitrite ABC transporter | NRT | Phormidium laminosum |
| periplasmic nitrate/nitrite binding subunit of NRT | NrtA | Phormidium laminosum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phormidium laminosum | truncated soluble form of NrtA at pH 5.5 | 6 | 5 |
| Phormidium laminosum | His-tagged NrtA1 at pH 5.5 | 5.9 | 5.1 |
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