Literature summary for 7.3.2.3 extracted from

Zolotarev, A.S.; Unnikrishnan, M.; Shmukler, B.E.; Clark, J.S.; Vandorpe, D.H.; Grigorieff, N.; Rubin, E.J.; Alper, S.L.
Increased sulfate uptake by E. coli overexpressing the SLC26-related SulP protein Rv1739c from Mycobacterium tuberculosis (2008), Comp. Biochem. Physiol. A, 149, 255-266.

Cloned(Commentary)

Cloned (Comment)	Organism
inducible expression of Rv1739c in an Escherichia coli strain EWP44 lacking CysA does not increase sulfate uptake by intact cells. Expression of Rv1739c in a Mycobacterium bovis BCG strain lacking the ABC sulfate permease subunit CysA cannot complement sulfate auxotrophy. Induction of Rv1739c expression in Escherichia coli increases bacterial uptake of sulfate, but not Cl-, formate, or oxalate. Sulfate uptake is also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain	Mycobacterium tuberculosis

Cloned (Comment)

Organism

inducible expression of Rv1739c in an Escherichia coli strain EWP44 lacking CysA does not increase sulfate uptake by intact cells. Expression of Rv1739c in a Mycobacterium bovis BCG strain lacking the ABC sulfate permease subunit CysA cannot complement sulfate auxotrophy. Induction of Rv1739c expression in Escherichia coli increases bacterial uptake of sulfate, but not Cl-, formate, or oxalate. Sulfate uptake is also increased by overexpression of the Rv1739c transmembrane domain, but not of the cytoplasmic C-terminal STAS domain

Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
additional information	mutation to serine of the three cysteine residues of Rv1739c does not affect magnitude, pH-dependence, or pharmacology of sulfate uptake	Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism
carbonyl cyanide 3-chloro-phenylhydrazone	-	Mycobacterium tuberculosis
additional information	no inhibition by bicarbonate, nitrate, or phosphate, or by the anion transport inhibitor NS3623	Mycobacterium tuberculosis
N-ethylmaleimide	-	Mycobacterium tuberculosis
selenate	-	Mycobacterium tuberculosis
sulfite	-	Mycobacterium tuberculosis
thiosulfate	-	Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	sulfate	pH 6.0, recombinant enzyme	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	Na+-independent sulfate uptake	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Mycobacterium tuberculosis	facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter	?	-	?
additional information	Mycobacterium tuberculosis H37Rv	facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	gene Rv1739c	-
Mycobacterium tuberculosis H37Rv	-	gene Rv1739c	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + H2O + sulfate/out	facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter	Mycobacterium tuberculosis	ADP + phosphate + sulfate/in	-	?
ATP + H2O + sulfate/out	facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter	Mycobacterium tuberculosis H37Rv	ADP + phosphate + sulfate/in	-	?
additional information	facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter	Mycobacterium tuberculosis	?	-	?
additional information	Na+-independent sulfate uptake	Mycobacterium tuberculosis	?	-	?
additional information	facilitation of bacterial sulfate uptake by Rv1739c requires CysA and its associated sulfate permease activity, thus Rv1739c may be a CysTWA-dependent sulfate transporter	Mycobacterium tuberculosis H37Rv	?	-	?
additional information	Na+-independent sulfate uptake	Mycobacterium tuberculosis H37Rv	?	-	?

Synonyms

Synonyms	Comment	Organism
Rv1739c	-	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Mycobacterium tuberculosis

pH Range

pH Minimum	pH Maximum	Comment	Organism
5	8	no activity below pH 5.0 and above pH 8.0	Mycobacterium tuberculosis