Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)-pGro7/GroEL cells | Clostridium perfringens |
Crystallization (Comment) | Organism |
---|---|
in complex with phosphate, sitting drop vapor diffusion method, using 0.1 M sodium acetate, 0.2 M zinc acetate pH 4.5, and 10% (w/v) PEG 3000 | Clostridium perfringens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | Clostridium perfringens | - |
ADP + phosphate + phosphate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium perfringens | A0A0H2YSI2 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap nickel affinity column chromatography and Superdex 75 gel filtration | Clostridium perfringens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + arsenate/out | the enzyme has about 150fold lower affinity for arsenate compared to phosphate | Clostridium perfringens | ADP + phosphate + arsenate/in | - |
? | |
ATP + H2O + phosphate/out | - |
Clostridium perfringens | ADP + phosphate + phosphate/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
high affinity phosphate-binding protein | - |
Clostridium perfringens |
PBP-1 | - |
Clostridium perfringens |