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Literature summary for 7.3.2.1 extracted from

  • Wu, Z.; Zhao, J.; Gao, R.; Hu, G.; Gai, J.; Xu, G.; Xing, H.
    Molecular cloning, characterization and expression analysis of two members of the Pht1 family of phosphate transporters in Glycine max (2011), PLoS ONE, 6, e19752.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
GmPT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in yeast MB192 mutant cells, expression as GFP-tagged protein in onion epidermal cells from chimeric gene CaMV35S-GmPT1 introduced into the cells by a particle bombardment system Glycine max
GmPT2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in yeast MB192 mutant cells, expression as GFP-tagged protein in onion epidermal cells from chimeric gene CaMV35S-GmPT1 introduced into the cells by a particle bombardment system Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
Glycine max 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Glycine max as phosphate/proton cotransport mechanism ADP + phosphate + phosphate/in
-
?
ATP + H2O + phosphate/out Glycine max gantai as phosphate/proton cotransport mechanism ADP + phosphate + phosphate/in
-
?

Organism

Organism UniProt Comment Textmining
Glycine max C0LZ80 GmPT2
-
Glycine max F5A0U7 GmPT1
-
Glycine max gantai C0LZ80 GmPT2
-
Glycine max gantai F5A0U7 GmPT1
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Glycine max
-
seedling
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out as phosphate/proton cotransport mechanism Glycine max ADP + phosphate + phosphate/in
-
?
ATP + H2O + phosphate/out as phosphate/proton cotransport mechanism Glycine max gantai ADP + phosphate + phosphate/in
-
?

Synonyms

Synonyms Comment Organism
GmPT1
-
Glycine max
GmPT2
-
Glycine max
Pi transporter
-
Glycine max

General Information

General Information Comment Organism
metabolism phosphate transporter mechanism in the plant cell, overview. When the supply of phosphate is limited, plants grow more roots, increase the rate of phosphate uptake by roots from soil solution, retranslocate phosphate from older leaves, and deplete the vacuolar stores of phosphate. There is also significant retranslocation of phosphate in the phloem from older leaves to the growing shoot and from the shoot to the root Glycine max
physiological function GmPT1 is a low-affinity phosphate transporter, the enzyme is important in phosphate uptake by roots and translocation within the plant, presumed to occur via a phosphate/proton cotransport mechanism Glycine max
physiological function GmPT2 is a low-affinity phosphate transporter, the enzyme is important in phosphate uptake by roots and translocation within the plant, presumed to occur via a phosphate/proton cotransport mechanism Glycine max