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Literature summary for 7.3.2.1 extracted from

  • Sun, S.; Gu, M.; Cao, Y.; Huang, X.; Zhang, X.; Ai, P.; Zhao, J.; Fan, X.; Xu, G.
    A constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in Pi-replete rice (2012), Plant Physiol., 159, 1571-1581.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
OsPT1 semi-quantitative RT-PCR and real time quantitative RT-PCR expression analyses Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information transgenic plants of OsPT1 overexpression lines and RNA-interference knockdown lines contain significantly higher and lower phosphate concentration, respectively, respectively, compared to that of wild-type control. OsPT1 is able to complement the nH+/phosphate co-transporter activities in a yeast mutant MB192 strain defective in phosphate-uptake. No significant difference of phosphate concentration in the roots of OsPT1-Ox1, OsPT1-Ri1 and wild-type irrespective of phosphate supply levels Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information OsPT1 follows Michaelis-Menten kinetics Oryza sativa
0.177
-
phosphate/out pH and temperature not specified in the publication Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Oryza sativa 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Oryza sativa
-
ADP + phosphate + phosphate/in
-
?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
epidermal cell
-
Oryza sativa
-
leaf young Oryza sativa
-
additional information the expression pattern of OsPT1 is constitutive independent of phosphate supply, expression analysis of OsPT1 by semi-quantitative RT-PCR Oryza sativa
-
root roots and the root-shoot junctions, expression in epidermal, cortical, and stele cells, OsPht1;1 is one of the 13 Pht1 Pi transporters in rice, expressed abundantly and constitutively in various cell types of both roots and shoots independent of phosphate supply condition Oryza sativa
-
root hair
-
Oryza sativa
-
shoot OsPht1;1is one of the 13 Pht1 Pi transporters in rice, expressed abundantly and constitutively in various cell types of both roots and shoots independent of phosphate supply condition Oryza sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out
-
Oryza sativa ADP + phosphate + phosphate/in
-
?

Synonyms

Synonyms Comment Organism
OsPht1;1
-
Oryza sativa
OsPT1
-
Oryza sativa
OsPT4
-
Oryza sativa
OsPT8
-
Oryza sativa
Pi transporter
-
Oryza sativa

Expression

Organism Comment Expression
Oryza sativa OsPT1 expression is strongly enhanced by mutation of phosphate over-accumulator 2, OsPHO2, but not by phosphate starvation response 2, OsPHR2. OsPT8 and OsPT4 are significantly and moderately upregulated, respectively, in OsPT1 overexpressing plants up

General Information

General Information Comment Organism
evolution OsPT1 belongs to the Pht1 family, like OsPT8 and OsPT4 Oryza sativa
malfunction over-expression or knockdown of OsPT1 cause altered phosphate concentration and uptake rate and distribution in phosphate-replete rice, as well as Pi uptake-elicited changes in the root cell membrane potential. Changes in expression affects root growth and root hair development Oryza sativa
additional information OsPT1 expression is not significantly regulated by phosphate-supply level in the transgenic rice plants. Expression of OsPT1 is upregulated in rice phosphate accumulator mutant (ospho2) and not altered in phosphate starvation responsive mutant (osphr2) Oryza sativa
physiological function constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-repleted rice plants Oryza sativa