Cloned (Comment) | Organism |
---|---|
OsPT1 semi-quantitative RT-PCR and real time quantitative RT-PCR expression analyses | Oryza sativa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | transgenic plants of OsPT1 overexpression lines and RNA-interference knockdown lines contain significantly higher and lower phosphate concentration, respectively, respectively, compared to that of wild-type control. OsPT1 is able to complement the nH+/phosphate co-transporter activities in a yeast mutant MB192 strain defective in phosphate-uptake. No significant difference of phosphate concentration in the roots of OsPT1-Ox1, OsPT1-Ri1 and wild-type irrespective of phosphate supply levels | Oryza sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | OsPT1 follows Michaelis-Menten kinetics | Oryza sativa | |
0.177 | - |
phosphate/out | pH and temperature not specified in the publication | Oryza sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Oryza sativa | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | Oryza sativa | - |
ADP + phosphate + phosphate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epidermal cell | - |
Oryza sativa | - |
leaf | young | Oryza sativa | - |
additional information | the expression pattern of OsPT1 is constitutive independent of phosphate supply, expression analysis of OsPT1 by semi-quantitative RT-PCR | Oryza sativa | - |
root | roots and the root-shoot junctions, expression in epidermal, cortical, and stele cells, OsPht1;1 is one of the 13 Pht1 Pi transporters in rice, expressed abundantly and constitutively in various cell types of both roots and shoots independent of phosphate supply condition | Oryza sativa | - |
root hair | - |
Oryza sativa | - |
shoot | OsPht1;1is one of the 13 Pht1 Pi transporters in rice, expressed abundantly and constitutively in various cell types of both roots and shoots independent of phosphate supply condition | Oryza sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | - |
Oryza sativa | ADP + phosphate + phosphate/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
OsPht1;1 | - |
Oryza sativa |
OsPT1 | - |
Oryza sativa |
OsPT4 | - |
Oryza sativa |
OsPT8 | - |
Oryza sativa |
Pi transporter | - |
Oryza sativa |
Organism | Comment | Expression |
---|---|---|
Oryza sativa | OsPT1 expression is strongly enhanced by mutation of phosphate over-accumulator 2, OsPHO2, but not by phosphate starvation response 2, OsPHR2. OsPT8 and OsPT4 are significantly and moderately upregulated, respectively, in OsPT1 overexpressing plants | up |
General Information | Comment | Organism |
---|---|---|
evolution | OsPT1 belongs to the Pht1 family, like OsPT8 and OsPT4 | Oryza sativa |
malfunction | over-expression or knockdown of OsPT1 cause altered phosphate concentration and uptake rate and distribution in phosphate-replete rice, as well as Pi uptake-elicited changes in the root cell membrane potential. Changes in expression affects root growth and root hair development | Oryza sativa |
additional information | OsPT1 expression is not significantly regulated by phosphate-supply level in the transgenic rice plants. Expression of OsPT1 is upregulated in rice phosphate accumulator mutant (ospho2) and not altered in phosphate starvation responsive mutant (osphr2) | Oryza sativa |
physiological function | constitutive expressed phosphate transporter, OsPht1;1, modulates phosphate uptake and translocation in phosphate-repleted rice plants | Oryza sativa |