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Literature summary for 7.3.2.1 extracted from

  • Irigoyen, S.; Karlsson, P.M.; Kuruvilla, J.; Spetea, C.; Versaw, W.K.
    The sink-specific plastidic phosphate transporter PHT4;2 influences starch accumulation and leaf size in Arabidopsis (2011), Plant Physiol., 157, 1765-1777.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene pht4;2, DNA and amino acid sequence determination and analysis, genetic organization, PHT4;2 is located adjacent to DEETIOLATED2 (DET2) on chromosome 2 (loci At2g38060 and At2g38050, respectively), with 550 bp between the PHT4;2 stop codon and the DET2 translation start site, quantitative RT-PCR expression analysis, recombinant expression of the processed enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Arabidopsis thaliana 9507
-
plastid root Arabidopsis thaliana 9536
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Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
x * 35000, SDS-PAGE, x * 50500, about, processed enzyme, sequence calculation Arabidopsis thaliana
50500
-
x * 35000, SDS-PAGE, x * 50500, about, processed enzyme, sequence calculation Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q7XJR2 PHT4;2; gene pht4;2
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-
additional information PHT4;2 is expressed in multiple sink organs but is nearly restricted to roots during vegetative growth Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE, x * 50500, about, processed enzyme, sequence calculation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
PHT4;2
-
Arabidopsis thaliana
plastid-localized Pi transporter
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction two independent T-DNA insertion lines from the SALK collection lacking a functional PHT4;2 allele, i.e. pht4;2-1 and pht4;2-2 mutant plants show altered activity and increased biomass due to increased leaf sizes, as well as altered starch accumulation, compared to the wild-type enzyme, phenotypes, overview Arabidopsis thaliana
physiological function PHT4;2 contributes to phosphate transport in isolated root plastids Arabidopsis thaliana