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Literature summary for 7.3.2.1 extracted from

  • Preuss, C.P.; Huang, C.Y.; Tyerman, S.D.
    Proton-coupled high-affinity phosphate transport revealed from heterologous characterization in Xenopus of barley-root plasma membrane transporter, HvPHT1;1 (2011), Plant Cell Environ., 34, 681-689.
    View publication on PubMed
Search for more literature for 7.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of HvPHT1;1 in Allium cepa epidermal cell plasma membranes from gateway-enabled pMDC83 vector with HvPHT1;1 being placed in-frame, upstream of the mGFP6 gene Hordeum vulgare

Protein Variants

Protein Variants Comment Organism
additional information HvPHT1;1 activity analysis in Xenopus laevis oocytes via cRNA-injection, low external sodium concentration is critical for phosphate transporter characterization in Xenopus laevis oocytes Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information negative voltage Michaelis-Menten kinetics data, overview Hordeum vulgare
0.0019
-
 phosphate/out pH 7.5, temperature not specified in the publication Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information PHT1;1 subcellular localization study, overview Hordeum vulgare
-
-
plasma membrane
-
 Hordeum vulgare 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Hordeum vulgare proton-coupled phosphate transport ADP + phosphate + phosphate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare Q8H6E0 cv. Clipper and cv. Sahara
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information cell-specific expression pattern of PHT1;1 Hordeum vulgare
-
root PHT1;1 is highly expressed in root segments with dense hairs and less root tips Hordeum vulgare
-
root hair
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out
-
 Hordeum vulgare ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out proton-coupled phosphate transport Hordeum vulgare ADP + phosphate + phosphate/in
-
 ?
additional information HvPHT1;1 shows preferential selectivity for phosphate and arsenate, but no transport of the other oxyanions SO42- and NO3- Hordeum vulgare ?
-
 ?

Synonyms

Synonyms Comment Organism
HvPHT1;1
-
 Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7.5 assay at Hordeum vulgare

Expression

Organism Comment Expression
Hordeum vulgare the HvPHT1;1 gene is largely induced by phosphate starvation up

General Information

General Information Comment Organism
physiological function HvPHT1;1 is a high-affinity phosphate transporter involved in uptake of phosphate from soil solution under low phosphate conditions Hordeum vulgare