Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | in yeast, several plasma membrane nutrient transporters are inactivated and targeted to the vacuole in response to changes in the availability of nutrients | Saccharomyces cerevisiae | - |
- |
plasma membrane | - |
Saccharomyces cerevisiae | 5886 | - |
vacuole | upon phosphate starvation, these low-affinity phosphate transporters, e.g. Pho87 and Pho90, are endocytosed and targeted to the vacuole. For Pho87, this process strictly depends on SPL2, another Pho4-dependent gene that encodes a protein known to interact with the N-terminal SPX domain of the transporter. In contrast, the vacuolar targeting of Pho90 upon phosphate starvation is independent of both Pho4 and Spl2, although it still requires its SPX domain. Both Pho87 and Pho90 are also targeted to the vacuole upon carbon-source starvation or upon treatment with rapamycin, which mimics nitrogen starvation, responses are independent of PHO pathway signalling requiring only the transporter N-terminal SPX domain. Spl2 mediates phosphate-starvation-induced vacuolar targeting of Pho87, but not of Pho90 | Saccharomyces cerevisiae | 5773 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90 | ? | - |
? | |
additional information | Saccharomyces cerevisiae BY4741 | Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Saccharomyces cerevisiae BY4741 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90 | Saccharomyces cerevisiae | ? | - |
? | |
additional information | Spl2 interacts with the N-terminal SPX domain of Pho87 and Pho90 | Saccharomyces cerevisiae BY4741 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
high-affinity phosphate transporter | - |
Saccharomyces cerevisiae |
low-affinity phosphate transporter | - |
Saccharomyces cerevisiae |
Pho84 | - |
Saccharomyces cerevisiae |
Pho87 | - |
Saccharomyces cerevisiae |
Pho90 | - |
Saccharomyces cerevisiae |
phosphate transporter | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Saccharomyces cerevisiae |
Organism | Comment | Expression |
---|---|---|
Saccharomyces cerevisiae | transcription of PHO87 and PHO90, encoding the low-affinity H+/phosphate transport system, is independent of phosphate status | additional information |
Saccharomyces cerevisiae | PHO84, encoding the high-affinity H+/phosphate symporter, expression is activated by the Pho4 transcription factor in phosphate-starved cells as part of the PHO signalling pathway | up |
General Information | Comment | Organism |
---|---|---|
malfunction | the pho84DELTA strain is unable to rapidly reverse phosphate-starvation responses. Deletion of END3, encoding an essential component for endocytosis, which prevents the internalization of Pho87 and, as a consequence, leads to the stabilization of this phosphate transporter at the plasma membrane under phosphate-starving conditions, endocytosis of Pho90 is only prevented in the mutant strain lacking End3 and not in the strains lacking Pho4 or Spl2 | Saccharomyces cerevisiae |
metabolism | upon phosphate starvation, these low-affinity phosphate transporters, e.g. Pho87 and Pho90, are endocytosed and targeted to the vacuole. For Pho87, this process strictly depends on SPL2, another Pho4-dependent gene that encodes a protein known to interact with the N-terminal SPX domain of the transporter | Saccharomyces cerevisiae |
additional information | the SPX domain is essential for vacuolar targeting of Pho87 and Pho90 in response to glucose starvation and rapamycin treatment | Saccharomyces cerevisiae |
physiological function | Pho87 and Pho90 are low-affinity phosphate transporters having differential roles. They represent non-redundant phosphate transporters, which are tuned by the integration of multiple nutrient signalling mechanisms in order to adjust phosphate-transport capacity to the general nutritional status of the environment. Pho90 is the most important phosphate transporter under high phosphate conditions in the absence of a high-affinity phosphate-transport system. Pho84 is the major phosphate transporter in Saccharomyces cerevisiae involved in rapid phosphate signalling | Saccharomyces cerevisiae |