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Literature summary for 7.3.2.1 extracted from

  • Ruiz-Pavon, L.; Karlsson, P.M.; Carlsson, J.; Samyn, D.; Persson, B.; Persson, B.L.; Spetea, C.
    Functionally important amino acids in the Arabidopsis thylakoid phosphate transporter: homology modeling and site-directed mutagenesis (2010), Biochemistry, 49, 6430-6439.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
D228E drastic increase in expression compared to wild-type, mutant shows no transport activity Arabidopsis thaliana
D382A Km: (phosphate) 0.057 mM (in the presence of 25 mM NaCl), 0.49 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity Arabidopsis thaliana
D382E Km: (phosphate) 0.046 mM (in the presence of 25 mM NaCl), 0.73 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity Arabidopsis thaliana
D382N mutant shows no transport activity Arabidopsis thaliana
R120K Km: (phosphate) 0.161 mM (in the presence of 25 mM NaCl), 1.48 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 250 nmol/mg of protein/h, results indicate that Arg-120 may be important for binding and translocation of the substrate Arabidopsis thaliana
R201K Km: (phosphate) 0.214 mM (in the presence of 25 mM NaCl), 2.29 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 100 nmol/mg of protein/h, results indicate that Arg-201 may be important for binding and translocation of the substrate Arabidopsis thaliana
R228K drastic increase in expression compared to wild-type, Km: (phosphate) 0.103 mM (in the presence of 25 mM NaCl), 1.99 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 274 nmol/mg of protein/h, drastic increase in expression compared to wild-type, results indicate that Arg-228 may participate in interactions associated with protein conformational changes required for full transport activity Arabidopsis thaliana
S124A Km: (phosphate) 0.0448 mM (in the presence of 25 mM NaCl), 0.27 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 47 nmol/mg of protein/h, results indicate that Ser-124 may function as a transient binding site for Na+ ions in close proximity to the periplasmic side Arabidopsis thaliana
S124T Km: (phosphate) 0.109 mM (in the presence of 25 mM NaCl), 0.88 mM (in the presence of various concentrations of NaCl), Vmax (adjusted to the protein expression levels): 254 nmol/mg of protein/h, results indicate that Ser-124 may function as a transient binding site for Na+ ions in close proximity to the periplasmic side Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0448
-
 phosphate mutant S124A, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 47 nmol/mg of protein/h Arabidopsis thaliana
0.0462
-
 phosphate mutant D382E, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 96 nmol/mg of protein/h Arabidopsis thaliana
0.0572
-
 phosphate mutant D382A, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 156 nmol/mg of protein/h Arabidopsis thaliana
0.077
-
 phosphate wild-type, pH 6.5, 25°C, in the presence of 25 mM NaCl Arabidopsis thaliana
0.103
-
 phosphate mutant R228K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 274 nmol/mg of protein/h Arabidopsis thaliana
0.109
-
 phosphate mutant S124T, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 254 nmol/mg of protein/h Arabidopsis thaliana
0.161
-
 phosphate mutant R120K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 250 nmol/mg of protein/h Arabidopsis thaliana
0.214
-
 phosphate mutant R201K, pH 6.5, 25°C, in the presence of 25 mM NaCl, Vmax (adjusted to the protein expression levels): 100.7 nmol/mg of protein/h Arabidopsis thaliana
0.27
-
 phosphate mutant S124A, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 62 nmol/mg of protein/h Arabidopsis thaliana
0.49
-
 phosphate mutant D382A, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 119 nmol/mg of protein/h Arabidopsis thaliana
0.73
-
 phosphate mutant D382E, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 132 nmol/mg of protein/h Arabidopsis thaliana
0.84
-
 phosphate wild-type, pH 6.5, 25°C, in the presence of various concentrations of NaCl Arabidopsis thaliana
0.88
-
 phosphate mutant S124T, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 222 nmol/mg of protein/h Arabidopsis thaliana
1.48
-
 phosphate mutant R120K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 125 nmol/mg of protein/h Arabidopsis thaliana
1.99
-
 phosphate mutant R228K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 221 nmol/mg of protein/h Arabidopsis thaliana
2.29
-
 phosphate mutant R201K, pH 6.5, 25°C, in the presence of various concentrations of NaCl, Vmax (adjusted to the protein expression levels): 126 nmol/mg of protein/h Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
thylakoid membrane
-
 Arabidopsis thaliana 42651
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ 25 mM Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 SDS-PAGE Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out
-
 Arabidopsis thaliana ADP + phosphate + phosphate/in
-
 ?

Synonyms

Synonyms Comment Organism
anion transporter 1
-
 Arabidopsis thaliana
ANTR1
-
 Arabidopsis thaliana
thylakoid phosphate transporter
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 assay at Arabidopsis thaliana