Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phosphate-limitation induces expression of phnD and pstS | Mycolicibacterium smegmatis |
Cloned (Comment) | Organism |
---|---|
pst and phn operons, the Phn system is encoded by a three-gene operon, DNA and amino acid sequence determinations and analysis, genomic organization, expression analyis of pstS and phnD, overview | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of deletion mutants, mutation of either pstS and phnD leads to impaired cell growth in minimal medium, the cells are only viable in high phosphate concentrated medium | Mycolicibacterium smegmatis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arsenate | strong inhibition | Mycolicibacterium smegmatis | |
methylphosphonate | competitive, partial inhibition of phosphate transport activity, overview | Mycolicibacterium smegmatis | |
phosphite | competitive, partial inhibition of phosphate transport activity, overview | Mycolicibacterium smegmatis | |
phosphonate | competitive, partial inhibition of phosphate transport activity, overview | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | phosphate transport kinetics in whole wild-type and mutant cells, overview | Mycolicibacterium smegmatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mycolicibacterium smegmatis | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | Mycolicibacterium smegmatis | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | Mycolicibacterium smegmatis SG34 | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | ADP + phosphate + phosphate/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
genes pstS and phnD | - |
Mycolicibacterium smegmatis SG34 | - |
genes pstS and phnD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + phosphate/out | - |
Mycolicibacterium smegmatis | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | Mycolicibacterium smegmatis | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | - |
Mycolicibacterium smegmatis SG34 | ADP + phosphate + phosphate/in | - |
? | |
ATP + H2O + phosphate/out | the organism possesses two different high-affinity phosphate ABC transporter systems, the Pst system, with isozymes PstS1 and PstS2, and the Phn system, overview | Mycolicibacterium smegmatis SG34 | ADP + phosphate + phosphate/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
high-affinity phosphate ABC-transporter | - |
Mycolicibacterium smegmatis |
Phn | - |
Mycolicibacterium smegmatis |
Pst | - |
Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Mycolicibacterium smegmatis |