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Literature summary for 7.3.2.1 extracted from

  • Sarin, J.; Aggarwal, S.; Chaba, R.; Varshney, G.C.; Chakraborti, P.K.
    B-subunit of phosphate-specific transporter from Mycobacterium tuberculosis is a thermostable ATPase (2001), J. Biol. Chem., 276, 44590-44597.
    View publication on PubMed
Search for more literature for 7.3.2.1

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D188K no ATP hydrolyzing ability Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
5'-p-fluorosulfonylbenzoyl adenosine pH 7.4, 30 min at 30°C, 1-3 mM Mycobacterium tuberculosis
guanidine hydrochloride 5 min at 100°C, 8000 mM, significant reduction in the activity Mycobacterium tuberculosis
Mn2+ at room temperature Mycobacterium tuberculosis
additional information no inhibition with sodium azide, sodium orthovanadate and N-ethylmaleimide Mycobacterium tuberculosis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0239
-
 ATP at 37 °C, pH 7.5 Escherichia coli
0.0715
-
 ATP at 37 °C, pH 7.5 Mycobacterium tuberculosis
0.13
-
 CTP at 37 °C, pH 7.5 Mycobacterium tuberculosis
0.211
-
 GTP at 37 °C, pH 7.5 Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
inclusion body
-
 Escherichia coli 16234
-
inclusion body
-
 Mycobacterium tuberculosis 16234
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no activation with Ca2+, Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
 PstB is the ATP-binding component, 2 * 30500, SDS-PAGE Mycobacterium tuberculosis
69300
-
 gel filtration chromatography Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + phosphate/out Escherichia coli ATP-consuming import of phosphate ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out Mycobacterium tuberculosis ATP-consuming import of phosphate ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out Mycobacterium tuberculosis H37Rv ATP-consuming import of phosphate ADP + phosphate + phosphate/in
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Mycobacterium tuberculosis
-
-
-
Mycobacterium tuberculosis H37Rv
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Escherichia coli
no modification
-
 Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment) Organism
by gel filtration chromatography using Superdex 200 column Escherichia coli
by gel permeation chromatography using Superdex 200 column Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + phosphate/out
-
 Escherichia coli ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out
-
 Mycobacterium tuberculosis ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out ATP-consuming import of phosphate Escherichia coli ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out ATP-consuming import of phosphate Mycobacterium tuberculosis ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + phosphate/in
-
 ?
ATP + H2O + phosphate/out ATP-consuming import of phosphate Mycobacterium tuberculosis H37Rv ADP + phosphate + phosphate/in
-
 ?
CTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis CDP + phosphate + phosphate/in
-
 ?
CTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis H37Rv CDP + phosphate + phosphate/in
-
 ?
GTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis GDP + phosphate + phosphate/in
-
 ?
GTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis H37Rv GDP + phosphate + phosphate/in
-
 ?

Subunits

Subunits Comment Organism
dimer PstB is the ATP-binding component, 2 * 30500, SDS-PAGE Mycobacterium tuberculosis
More PstB is the ATP binding component Escherichia coli

Synonyms

Synonyms Comment Organism
phosphate-specific transporter
-
 Escherichia coli
phosphate-specific transporter
-
 Mycobacterium tuberculosis
Pst
-
 Escherichia coli
Pst
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
24 80 pH 7.5, for 5 min, enzyme is heat-resistant Mycobacterium tuberculosis
37
-
 pH 7.5, for 5 min, substantial loss in the enzyme activity at temperatures above 37°C Escherichia coli
80
-
 pH 7.5 Mycobacterium tuberculosis
80
-
 pH 7.5, between 30 and 60 min of heat, maximum loss of enzyme activity, 60% of initial value Escherichia coli
80
-
 pH 7.5, incubating for 15 min, proteins are precipitated Escherichia coli
80
-
 pH 7.5, thermal denaturation is evident within 5 min Escherichia coli
80
-
 pH 7.5,incubating for 15 min, hardly shows any aggregation and for 1 h, loss 11% of its initial enzyme activity Mycobacterium tuberculosis

pH Range

pH Minimum pH Maximum Comment Organism
5 10
-
 Mycobacterium tuberculosis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.4
-
 5'-p-fluorosulfonylbenzoyl adenosine pH 7.4, at 30°C Mycobacterium tuberculosis