Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.4.5 extracted from

  • Kress, D.; Bruegel, D.; Schall, I.; Linder, D.; Buckel, W.; Essen, L.O.
    An asymmetric model for Na+-translocating glutaconyl-CoA decarboxylases (2009), J. Biol. Chem., 284, 28401-28409.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.4.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells [Clostridium] symbiosum

Crystallization (Commentary)

Crystallization (Comment) Organism
co-crystallization of the decarboxylase subunit GcdA with the substrate glutaconyl-CoA, the product crotonyl-CoA, and the substrate analogue glutaryl-CoA, sitting drop vapor diffusion method, using in 0.1 M sodium citrate, pH 5.6-6.5, 1 M ammonium phosphate, 27.5% (v/v) glycerol [Clostridium] symbiosum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 [Clostridium] symbiosum 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12000
-
 1 * 65000 + 1 * 35000 + 1 * 14000 + 1 * 12000, X-ray crystallography [Clostridium] symbiosum
14000
-
 1 * 65000 + 1 * 35000 + 1 * 14000 + 1 * 12000, X-ray crystallography [Clostridium] symbiosum
35000
-
 1 * 65000 + 1 * 35000 + 1 * 14000 + 1 * 12000, X-ray crystallography [Clostridium] symbiosum
65000
-
 1 * 65000 + 1 * 35000 + 1 * 14000 + 1 * 12000, X-ray crystallography [Clostridium] symbiosum
232000
-
 gel filtration [Clostridium] symbiosum

Organism

Organism UniProt Comment Textmining
[Clostridium] symbiosum B7TVP1 subunit A
-
[Clostridium] symbiosum HB25 B7TVP1 subunit A
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin Sepharose column chromatography, Ni-NTA affinity chromatography, and avidin-biotin affinity chromatography [Clostridium] symbiosum

Subunits

Subunits Comment Organism
heterotetramer 1 * 65000 + 1 * 35000 + 1 * 14000 + 1 * 12000, X-ray crystallography [Clostridium] symbiosum

Synonyms

Synonyms Comment Organism
GCD
-
 [Clostridium] symbiosum

Cofactor

Cofactor Comment Organism Structure
biotin
-
 [Clostridium] symbiosum

General Information

General Information Comment Organism
physiological function Gcd couples the biotin-dependent decarboxylation of glutaconyl-CoA with the generation of an electrochemical Na+ gradient [Clostridium] symbiosum