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Literature summary for 7.2.4.5 extracted from
- Biotin-dependent decarboxylases as bacterial sodium pumps: Purification and reconstitution of glutaconyl-CoA decarboxylase from Acidaminococcus fermentans (1986), Methods Enzymol., 125, 547-558.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| biotin | biotinyl enzyme | Peptoniphilus asaccharolyticus |  |
| biotin | biotinyl enzyme | Fusobacterium nucleatum | |
| biotin | biotinyl enzyme | [Clostridium] symbiosum | |
| biotin | biotinyl enzyme | Acidaminococcus fermentans | |
| Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Peptoniphilus asaccharolyticus | |
| Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Fusobacterium nucleatum | |
| Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | [Clostridium] symbiosum | |
| Brij 35 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Acidaminococcus fermentans | |
| Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Peptoniphilus asaccharolyticus | |
| Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Fusobacterium nucleatum | |
| Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | [Clostridium] symbiosum | |
| Triton X-100 | only Triton X-100 and Brij 35 are able to keep the enzyme in an active state at 20-30°C | Acidaminococcus fermentans | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Avidin | not: avidin biotin complex | Acidaminococcus fermentans | |
| Avidin | not: avidin biotin complex | [Clostridium] symbiosum | |
| Avidin | not: avidin biotin complex | Fusobacterium nucleatum | |
| Avidin | not: avidin biotin complex | Peptoniphilus asaccharolyticus | |
| deoxycholate | at 20-30°C | Acidaminococcus fermentans | |
| deoxycholate | at 20-30°C | [Clostridium] symbiosum | |
| deoxycholate | at 20-30°C | Peptoniphilus asaccharolyticus | |
| ethanol | - |
Acidaminococcus fermentans | |
| ethanol | - |
[Clostridium] symbiosum | |
| ethanol | - |
Fusobacterium nucleatum | |
| ethanol | - |
Peptoniphilus asaccharolyticus | |
| N-Alkanols | concentration required for complete inactivation decreases N-dodecyl-N-dimethylaminoxide at 20-30°C | Acidaminococcus fermentans | |
| N-ethylmaleimide | - |
Acidaminococcus fermentans | |
| N-ethylmaleimide | - |
[Clostridium] symbiosum | |
| N-ethylmaleimide | - |
Fusobacterium nucleatum | |
| N-ethylmaleimide | - |
Peptoniphilus asaccharolyticus | |
| SDS | at 20-30°C | Acidaminococcus fermentans | |
| SDS | at 20-30°C | [Clostridium] symbiosum | |
| SDS | at 20-30°C | Peptoniphilus asaccharolyticus | |
| Triton X-114 | at 20-30°C | Acidaminococcus fermentans | |
| Triton X-114 | at 20-30°C | [Clostridium] symbiosum | |
| Triton X-114 | at 20-30°C | Peptoniphilus asaccharolyticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Peptoniphilus asaccharolyticus | 16020 | - |
| membrane | - |
Fusobacterium nucleatum | 16020 | - |
| membrane | - |
[Clostridium] symbiosum | 16020 | - |
| membrane | - |
Acidaminococcus fermentans | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | activates, Km: 100 mM | Peptoniphilus asaccharolyticus | |
| Li+ | activates, Km: 100 mM | Fusobacterium nucleatum | |
| Li+ | activates, Km: 100 mM | [Clostridium] symbiosum | |
| Li+ | activates, Km: 100 mM | Acidaminococcus fermentans | |
| Na+ | Km: 1 mM | Acidaminococcus fermentans | |
| Na+ | stimulates | Peptoniphilus asaccharolyticus | |
| Na+ | stimulates | Fusobacterium nucleatum | |
| Na+ | stimulates | [Clostridium] symbiosum | |
| Na+ | stimulates | Acidaminococcus fermentans | |
| Na+ | activation by Na+ is inhibited by Li+ | Peptoniphilus asaccharolyticus | |
| Na+ | activation by Na+ is inhibited by Li+ | Fusobacterium nucleatum | |
| Na+ | activation by Na+ is inhibited by Li+ | [Clostridium] symbiosum | |
| Na+ | activation by Na+ is inhibited by Li+ | Acidaminococcus fermentans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
in sucrose density gradient centrifugation the activity is recovered in 2 peaks: MW 470000, possibly dimer and MW 250000, possibly monomer | Acidaminococcus fermentans |
| 35000 | - |
1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE | Acidaminococcus fermentans |
| 60000 | - |
1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE | Acidaminococcus fermentans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutaconyl-CoA + Na+[side 1] | Peptoniphilus asaccharolyticus | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | Fusobacterium nucleatum | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | [Clostridium] symbiosum | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | Acidaminococcus fermentans | - |
but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acidaminococcus fermentans | - |
ATCC 25085 | - |
| Fusobacterium nucleatum | - |
- |
- |
| Peptoniphilus asaccharolyticus | - |
- |
- |
| [Clostridium] symbiosum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acidaminococcus fermentans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, little loss of activity after 1 year | Acidaminococcus fermentans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Peptoniphilus asaccharolyticus | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Fusobacterium nucleatum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
[Clostridium] symbiosum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| 4-carboxybut-2-enoyl-CoA + Na+[side 1] | - |
Acidaminococcus fermentans | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | - |
Peptoniphilus asaccharolyticus | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | - |
Fusobacterium nucleatum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | - |
[Clostridium] symbiosum | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| glutaconyl-CoA + Na+[side 1] | - |
Acidaminococcus fermentans | but-2-enoyl-CoA + CO2 + Na+[side 2] | - |
? | |
| additional information | not: 3-methylglutaconyl-CoA | Peptoniphilus asaccharolyticus | ? | - |
? | |
| additional information | not: 3-methylglutaconyl-CoA | Fusobacterium nucleatum | ? | - |
? | |
| additional information | not: 3-methylglutaconyl-CoA | [Clostridium] symbiosum | ? | - |
? | |
| additional information | not: 3-methylglutaconyl-CoA | Acidaminococcus fermentans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | - |
Fusobacterium nucleatum |
| ? | 1 * 120000-140000, alpha, contains biotin + 1 * 60000, beta, + 1 * 35000, gamma, hydrophobic, SDS-PAGE | Acidaminococcus fermentans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Peptoniphilus asaccharolyticus |
| 25 | - |
assay at | Fusobacterium nucleatum |
| 25 | - |
assay at | [Clostridium] symbiosum |
| 25 | - |
assay at | Acidaminococcus fermentans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Peptoniphilus asaccharolyticus |
| 7 | - |
assay at | Fusobacterium nucleatum |
| 7 | - |
assay at | [Clostridium] symbiosum |
| 7 | - |
assay at | Acidaminococcus fermentans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| biotin | biotinyl enzyme | Peptoniphilus asaccharolyticus | |
| biotin | biotinyl enzyme | Fusobacterium nucleatum | |
| biotin | biotinyl enzyme | [Clostridium] symbiosum | |
| biotin | biotinyl enzyme | Acidaminococcus fermentans | |
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