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Literature summary for 7.2.4.5 extracted from

  • Beatrix, B.; Bendrat, K.; Rospert, S.; Buckel, W.
    The biotin-dependent sodium ion pump glutaconyl-CoA decarboxylase from Fusobacterium nucleatum (subsp. nucleatum) (1990), Arch. Microbiol., 154, 362-369.
    View publication on PubMed
Search for more literature for 7.2.4.5

Activating Compound

Activating Compound Comment Organism Structure
biotin biotinyl enzyme Peptoniphilus asaccharolyticus
biotin biotinyl enzyme Fusobacterium nucleatum
biotin biotinyl enzyme [Clostridium] symbiosum
biotin biotinyl enzyme Acidaminococcus fermentans
Phospholipid no stimulation [Clostridium] symbiosum
Phospholipid no stimulation Acidaminococcus fermentans
Phospholipid stimulates enzyme Fusobacterium nucleatum

General Stability

General Stability Organism
requires Na+ for stability Fusobacterium nucleatum
unstable in the presence of buffers containing potassium Fusobacterium nucleatum

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Peptoniphilus asaccharolyticus 16020
-
membrane
-
 Fusobacterium nucleatum 16020
-
membrane
-
 [Clostridium] symbiosum 16020
-
membrane
-
 Acidaminococcus fermentans 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Na+ required Peptoniphilus asaccharolyticus
Na+ required Fusobacterium nucleatum
Na+ required [Clostridium] symbiosum
Na+ required Acidaminococcus fermentans
Na+ Km: 1 mM Acidaminococcus fermentans
Na+ Km: 3 mM Fusobacterium nucleatum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
16000
-
 x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE Fusobacterium nucleatum
19000
-
 x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE Fusobacterium nucleatum
33000
-
 x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE Fusobacterium nucleatum
65000
-
 x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE Fusobacterium nucleatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutaconyl-CoA + Na+[side 1] Peptoniphilus asaccharolyticus
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1] Fusobacterium nucleatum
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1] [Clostridium] symbiosum
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1] Acidaminococcus fermentans
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
1-butanol concentration 2%, 50% loss of activity after 2 min Acidaminococcus fermentans
1-butanol concentration 1%, 50% loss of activity after 1 min in presence of 200 mM KCl, 50% loss of activity after 3 min in presence of 200 mM NaCl Fusobacterium nucleatum

Organism

Organism UniProt Comment Textmining
Acidaminococcus fermentans
-
 ATCC 25085
-
Fusobacterium nucleatum
-
 subsp. nucleatum
-
Peptoniphilus asaccharolyticus
-
-
-
[Clostridium] symbiosum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Peptoniphilus asaccharolyticus
-
 [Clostridium] symbiosum
-
 Acidaminococcus fermentans
subsp. nucleatum Fusobacterium nucleatum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Peptoniphilus asaccharolyticus
additional information
-
-
 Fusobacterium nucleatum
additional information
-
-
 [Clostridium] symbiosum
additional information
-
-
 Acidaminococcus fermentans

Storage Stability

Storage Stability Organism
-80°C, 24 h, 100 mM Na+, stable Fusobacterium nucleatum
4°C, 24 h, 100 mM Na+, 50% loss of activity Fusobacterium nucleatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Peptoniphilus asaccharolyticus but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Fusobacterium nucleatum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 [Clostridium] symbiosum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Acidaminococcus fermentans but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1]
-
 Peptoniphilus asaccharolyticus but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1]
-
 Fusobacterium nucleatum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1]
-
 [Clostridium] symbiosum but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1]
-
 Acidaminococcus fermentans but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

Subunits Comment Organism
?
-
 Acidaminococcus fermentans
? x * 65000 alpha, carboxytransferase + x * 33000, beta, carboxylyase + x * 19000, gamma, biotin carrier + x * 16000, probably carboxylyase, SDS-PAGE Fusobacterium nucleatum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min Peptoniphilus asaccharolyticus
37
-
 200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min Fusobacterium nucleatum
37
-
 200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min [Clostridium] symbiosum
37
-
 200 mM KCl: 50% loss of activity after 4 min, 200 mM NaCl: 10% loss of activity after 15 min Acidaminococcus fermentans

Cofactor

Cofactor Comment Organism Structure
biotin biotinyl enzyme Peptoniphilus asaccharolyticus
biotin biotinyl enzyme Fusobacterium nucleatum
biotin biotinyl enzyme [Clostridium] symbiosum
biotin biotinyl enzyme Acidaminococcus fermentans