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Literature summary for 7.2.4.5 extracted from

  • Haertel, U.; Eckel, E.; Koch, J.; Fuchs, G.; Linder, D.; Buckel, W.
    Purification of glutaryl-CoA dehydrogenase from Pseudomonas sp., an enzyme involved in the anaerobic degradation of benzoate (1993), Arch. Microbiol., 159, 174-181.
    View publication on PubMed
Search for more literature for 7.2.4.5

Inhibitors

Inhibitors Comment Organism Structure
glutaryl-CoA competitive to glutaconyl-CoA Pseudomonas sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3 5 glutaryl-CoA 28°C, pH 7.5 Pseudomonas sp.
6 11 glutaconyl-CoA 28°C, pH 7.5 Pseudomonas sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Pseudomonas sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 4 * 41000, SDS-PAGE Pseudomonas sp.
170000
-
 gel filtration Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glutaconyl-CoA + Na+[side 1] Pseudomonas sp.
-
 but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 enzyme also decarboxylates glutaryl-CoA
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxybut-2-enoyl-CoA + Na+[side 1]
-
 Pseudomonas sp. but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaconyl-CoA + Na+[side 1]
-
 Pseudomonas sp. but-2-enoyl-CoA + CO2 + Na+[side 2]
-
 ?
glutaryl-CoA + Na+[side 1]
-
 Pseudomonas sp. butyryl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41000, SDS-PAGE Pseudomonas sp.

Cofactor

Cofactor Comment Organism Structure
FAD 1.1 mol of FAD per subunit, Km: 0.00025 mM Pseudomonas sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0074
-
 glutaryl-CoA
-
 Pseudomonas sp.