BRENDA - Enzyme Database
show all sequences of 7.2.4.4

Purification and characterization of a cytoplasmic enzyme component of the Na+-activated malonate decarboxylase system of Malonomonas rubra: acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase

Hilbi, H.; Dimroth, P.; Arch. Microbiol. 162, 48-56 (1994)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Malonomonas rubra
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Malonomonas rubra
-
-
-
Purification (Commentary)
Commentary
Organism
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase and of whole enzyme complex
Malonomonas rubra
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
42
-
pH 6.0
Malonomonas rubra
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + H+
-
684745
Malonomonas rubra
acetate + CO2
-
-
-
?
additional information
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
684745
Malonomonas rubra
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
67000
-
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Malonomonas rubra
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
purification of subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase and of whole enzyme complex
Malonomonas rubra
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
42
-
pH 6.0
Malonomonas rubra
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
malonate + H+
-
684745
Malonomonas rubra
acetate + CO2
-
-
-
?
additional information
subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase catalyzes the transfer of acyl carrier protein from acetyl acyl carrier protein and malonate to yield malonyl acyl carrier protein and acetate. Malonate is thus activated on the enzyme by exchange for the catalytically important enzyme-bound acetyl thioester residues
684745
Malonomonas rubra
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 67000, SDS-PAGE, subunit acetyl-S-acyl carrier protein: malonate acyl carrier protein-SH transferase
Malonomonas rubra
Other publictions for EC 7.2.4.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
684746
Berg
The biotin protein MadF of the ...
Malonomonas rubra
Arch. Microbiol.
170
464-468
1998
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686491
Berg
Sequence of a gene cluster fro ...
Malonomonas rubra
Eur. J. Biochem.
245
103-115
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
685053
Berg
The acyl carrier protein of ma ...
Malonomonas rubra
Biochemistry
35
4689-4696
1996
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
687270
Micklefield
-
Stereochemical course of malon ...
Malonomonas rubra
J. Am. Chem. Soc.
117
1153-1154
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684745
Hilbi
Purification and characterizat ...
Malonomonas rubra
Arch. Microbiol.
162
48-56
1994
-
-
-
-
-
-
-
-
1
-
1
1
-
2
-
-
1
-
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
-
-
1
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684744
Hilbi
The malonate decarboxylase enz ...
Malonomonas rubra, Propionigenium modestum
Arch. Microbiol.
160
126-131
1993
-
-
-
-
-
-
1
-
4
-
-
-
-
2
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
4
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
686488
Hilbi
Malonate decarboxylase of Malo ...
Malonomonas rubra
Eur. J. Biochem.
207
117-123
1992
-
-
-
-
-
-
2
-
-
-
-
-
-
1
-
-
-
1
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-