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Literature summary for 7.2.4.3 extracted from

  • Bott, M.; Pfister, K.; Burda, P.; Kalbermatter, O.; Woehlke, G.; Dimroth, P.
    Methylmalonyl-CoA decarboxylase from Propionibacterium modestum. Cloning and sequencing of the structural genes and purification of the enzyme complex (1997), Eur. J. Biochem., 250, 590-599.
    View publication on PubMed
Search for more literature for 7.2.4.3

Cloned(Commentary)

Cloned (Comment) Organism
-
 Propionigenium modestum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 (S)-methylmalonyl-CoA
-
 Propionigenium modestum

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
13100
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
14200
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
17000
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
17500
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
35000
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
41200
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
56000
-
 x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum
56100
-
 x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] Propionigenium modestum the enzyme catalyzes the only energy-conserving step during succinate fermentation. The decarboxylation of (S)-methylmalonyl-CoA is coupled to the vectorial transport of Na+ across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for ATP synthesis propanoyl-CoA + CO2 + Na+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Propionigenium modestum
-
 DSM 2376
-

Purification (Commentary)

Purification (Comment) Organism
-
 Propionigenium modestum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Propionigenium modestum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2]
-
 Propionigenium modestum propanoyl-CoA + CO2 + Na+[side 2]
-
 ?
(S)-methylmalonyl-CoA + Na+[side 1] + H+[side 2] the enzyme catalyzes the only energy-conserving step during succinate fermentation. The decarboxylation of (S)-methylmalonyl-CoA is coupled to the vectorial transport of Na+ across the cytoplasmic membrane, thereby creating a sodium ion motive force that is used for ATP synthesis Propionigenium modestum propanoyl-CoA + CO2 + Na+[side 2]
-
 ?

Subunits

Subunits Comment Organism
? x * 56100, alpha, carboxyltransferase, + x * 41200, beta, carboxybiotin-carrier-protein decarboxylase, + x * 13100, gamma, biotin carrier protein, + x * 14200, delta, essential for the assembly of the complex, calculation of the MW from nucleotide sequence Propionigenium modestum
? x * 56000 + x * 35000 + x * 17500 + x * 17000, SDS-PAGE Propionigenium modestum