Literature summary for 7.2.2.9 extracted from

L�bben, M.; G�ldenhaupt, J.; Zoltner, M.; Deigweiher, K.; Haebel, P.; Urbanke, C.; Scheidig, A.J.
Sulfate acts as phosphate analog on the monomeric catalytic fragment of the CPx-ATPase CopB from Sulfolobus solfataricus (2007), J. Mol. Biol., 369, 368-385.

Cloned(Commentary)

Cloned (Comment)	Organism
the catalytic fragment CopB-B is expressed in Escherichia coli	Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystallization trials are performed at 18 °C by vapor diffusion using the hanging-drop technique, crystal structure of the catalytic fragment of a Sulfolobus solfataricus P-type ATPase, CopB-B, is determined with a 2.6 A resolution.In CopB-B crystals, a bound sulfate anion is identified at the phosphate-binding location. In solution state bound phosphate can be readily displaced by orthovanadate at submillimolar concentration as well as by sulfate at millimolar concentration. It is possible to assign the structure of the sulfate-bound phosphorylation domain of CopB-B to a state related to an enzyme/phosphate intermediate state of the catalytic cycle	Saccharolobus solfataricus

Crystallization (Comment)

Organism

crystallization trials are performed at 18 °C by vapor diffusion using the hanging-drop technique, crystal structure of the catalytic fragment of a Sulfolobus solfataricus P-type ATPase, CopB-B, is determined with a 2.6 A resolution.In CopB-B crystals, a bound sulfate anion is identified at the phosphate-binding location. In solution state bound phosphate can be readily displaced by orthovanadate at submillimolar concentration as well as by sulfate at millimolar concentration. It is possible to assign the structure of the sulfate-bound phosphorylation domain of CopB-B to a state related to an enzyme/phosphate intermediate state of the catalytic cycle

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	Q97UU7	CopB	-

Synonyms

Synonyms	Comment	Organism
CopB	-	Saccharolobus solfataricus
CPx-ATPase	-	Saccharolobus solfataricus

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Release 2023.1 (January 2023)