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Literature summary for 7.2.2.9 extracted from
- High yield heterologous expression of wild-type and mutant Cu+-ATPase (ATP7B, Wilson disease protein) for functional characterization of catalytic activity and serine residues undergoing copper-dependent phosphorylation (2009), J. Biol. Chem., 284, 21307-21316.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant myc-tagged ATP7Bs in COS1 cells using an adenoviral vector | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C983A/C985A | site-directed mutagenesis of CXC copper binding motif in the transmembrane copper site TM6, absence of alkali labile phosphoenzyme formation | Homo sapiens |
| D1027N | site-directed mutagenesis of the P domain, absence of alkali labile phosphoenzyme formation | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Bathocuproine disulfonate | a copper chelator, reduced ATP7B activity | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Cu2+/in | Homo sapiens | - |
ADP + phosphate + Cu2+/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | ATP7B phosphorylation with ATP occurs with diphasic kinetics and is totally copper-dependent | Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant myc-tagged ATP7B is isolated from the microsomal fraction of COS-1 cell homogenates | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.081 | - |
partially purifed recombinant ATP7B in microsomes, copper-dependent, steady-state ATPase, 37°C, pH 6.0, in presence of copper and copper chelator | Homo sapiens |
| 0.112 | - |
partially purifed recombinant ATP7B in microsomes, copper-dependent, steady-state ATPase, 37°C, pH 6.0 in presence of copper | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Cu2+/in | - |
Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
| ATP + H2O + Cu2+/in | ATP7B phosphorylation with ATP occurs with diphasic kinetics and is totally copper-dependent | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP7B | - |
Homo sapiens |
| Cu+-ATPase | - |
Homo sapiens |
| Wilson disease protein | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | ATP7B is a P-type ATPase required for copper homeostasis and related to Wilson disease of humans | Homo sapiens |
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