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Literature summary for 7.2.2.9 extracted from

  • Safaei, R.; Otani, S.; Larson, B.J.; Rasmussen, M.L.; Howell, S.B.
    Transport of cisplatin by the copper efflux transporter ATP7B (2008), Mol. Pharmacol., 73, 461-468.
    View publication on PubMed
Search for more literature for 7.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
expression in Spodoptera frugiperda Sf-9 cells Homo sapiens
infection of Sf9 cells with baculoviral constructs encoding wild-type ATP7B and a mutant variant unable to transport copper Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information CPA mutant analyzed, transmembrane transport function disabled by conversion of the transmembrane metal binding CPC motif to CPA Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cisplatin DDP, shown to inhibit the transport of copper, pH 4.6 Homo sapiens
Cu2+ shown to inhibit the transport of cisplatin Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu2+/in Homo sapiens
-
 ADP + phosphate + Cu2+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P35670
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 ATP-dependent accumulation of cisplatin indicated, cisplatin shown to induce acyl-phosphorylation of ATP7B but at slower rate than copper, Vmax value for cisplatin shown to be nearly 28fold lower than that for copper Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu2+/in
-
 Homo sapiens ADP + phosphate + Cu2+/out
-
 ?
ATP + H2O + Cu2+/in ATP7B shown to regulate cellular efflux of cisplatin (DDP), control of sensitivity to cytotoxic effects determined Homo sapiens ADP + phosphate + Cu2+/out
-
 ?

Synonyms

Synonyms Comment Organism
ATP7B
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.9
-
 transport assay at Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.6 6.9 ability of wild-type ATP7B to transport copper when exposed to 2 microM copper shown to be greater at pH 4.6 than at pH 6.9, ATP-dependent accumulation of cisplatin shown at 4.6 Homo sapiens