Cloned (Comment) | Organism |
---|---|
ATP7B expression in Sf9 insect cells, epitope-tagged ATP7B-HA56 variant generated and expressed in oocytes of Xenopus laevis, N-terminal and C-terminal deletions included to analyze delivery of ATP7B-HA56 from the membrane, wild-type ATP7B used as a control | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D1027A | inactivating mutation of catalytic Asp1027, surface targeting | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | targeting to plasma membrane shown, wild-type and recombinant protein | Homo sapiens | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | Homo sapiens | - |
ADP + phosphate + Cu2+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P35670 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | heterologous expression into Xenopus laevis | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
analysis of ATP7B at the cell surface by expression studies in intact cells, presence of ATP7B at the plasma membrane shown by electron microscopy, freeze-fracture experiments and surface luminescence measurements, delivery to the plasma membrane of oocytes unaffected by deletion of the N-terminal copper-binding domain or the inactivating mutation of catalytic Asp1027, surface targeting decreased for ATP7B variants with mutations in the ATP-binding site or in the copper-binding site, ligand-stabilized conformations important for ATP7B trafficking | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu2+/in | - |
Homo sapiens | ADP + phosphate + Cu2+/out | - |
? | |
ATP + H2O + Cu2+/in | transient delivery of the ATP7B- and copper-containing vesicles to the apical membrane analyzed | Homo sapiens | ADP + phosphate + Cu2+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP7B | - |
Homo sapiens |
Wilson disease protein | - |
Homo sapiens |