Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.2.9 extracted from

  • Loerinczi, E.; Tsivkovskii, R.; Haase, W.; Bamberg, E.; Lutsenko, S.; Friedrich, T.
    Delivery of the Cu-transporting ATPase ATP7B to the plasma membrane in Xenopus oocytes (2008), Biochim. Biophys. Acta, 1778, 896-906.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.9

Cloned(Commentary)

Cloned (Comment) Organism
ATP7B expression in Sf9 insect cells, epitope-tagged ATP7B-HA56 variant generated and expressed in oocytes of Xenopus laevis, N-terminal and C-terminal deletions included to analyze delivery of ATP7B-HA56 from the membrane, wild-type ATP7B used as a control Homo sapiens

Protein Variants

Protein Variants Comment Organism
D1027A inactivating mutation of catalytic Asp1027, surface targeting Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane targeting to plasma membrane shown, wild-type and recombinant protein Homo sapiens 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu2+/in Homo sapiens
-
 ADP + phosphate + Cu2+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P35670
-
-

Source Tissue

Source Tissue Comment Organism Textmining
oocyte heterologous expression into Xenopus laevis Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 analysis of ATP7B at the cell surface by expression studies in intact cells, presence of ATP7B at the plasma membrane shown by electron microscopy, freeze-fracture experiments and surface luminescence measurements, delivery to the plasma membrane of oocytes unaffected by deletion of the N-terminal copper-binding domain or the inactivating mutation of catalytic Asp1027, surface targeting decreased for ATP7B variants with mutations in the ATP-binding site or in the copper-binding site, ligand-stabilized conformations important for ATP7B trafficking Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu2+/in
-
 Homo sapiens ADP + phosphate + Cu2+/out
-
 ?
ATP + H2O + Cu2+/in transient delivery of the ATP7B- and copper-containing vesicles to the apical membrane analyzed Homo sapiens ADP + phosphate + Cu2+/out
-
 ?

Synonyms

Synonyms Comment Organism
ATP7B
-
 Homo sapiens
Wilson disease protein
-
 Homo sapiens