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Literature summary for 7.2.2.9 extracted from

  • DiDonato, M.; Hsu, H.F.; Narindrasorasak, S.; Que, L.; Sarkar, B.
    Copper-induced conformational changes in the N-terminal domain of the Wilson disease copper-transporting ATPase (2000), Biochemistry, 39, 1890-1896.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
enzyme is expressed using pGEX-4T-2, subcloned into pGEX-6P-2 and expressed in Escherichia coli strain BL21 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu2+/in Homo sapiens the copper-induced conformational changes could play an important role in the function and regulation of the ATPase in vivo ADP + phosphate + Cu2+/out
-
?
ATP + H2O + Cu2+/in Homo sapiens the Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death ADP + phosphate + Cu2+/out
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu2+/in each binding site ligates copper in the +1 oxidation state using two cysteine side chains with distorted linear geometry, both secondary and tertiary changes take place upon copper binding Homo sapiens ADP + phosphate + Cu2+/out
-
?
ATP + H2O + Cu2+/in the copper-induced conformational changes could play an important role in the function and regulation of the ATPase in vivo Homo sapiens ADP + phosphate + Cu2+/out
-
?
ATP + H2O + Cu2+/in the Wilson disease copper-transporting ATPase plays a critical role in the intracellular trafficking of copper. Mutations in this protein lead to the accumulation of a toxic level of copper in the liver, kidney, and brain followed by extensive tissue damage and death Homo sapiens ADP + phosphate + Cu2+/out
-
?

Subunits

Subunits Comment Organism
More the amino-terminal domain of the enzyme contains six repeats of the copper binding motif GMTCXXC Homo sapiens