Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Archaeoglobus fulgidus |
expression in Escherichia coli | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
C382A | the mutant exhibits reduced copper binding activity | Archaeoglobus fulgidus |
M711A | the mutant exhibits reduced copper binding activity | Archaeoglobus fulgidus |
S715A | the mutant exhibits reduced copper binding activity | Archaeoglobus fulgidus |
Y682A | the mutant exhibits reduced copper binding activity | Archaeoglobus fulgidus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Archaeoglobus fulgidus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu+ | the enzyme contains 2.2 Cu+ and 3.1 ions Cu+ per molecule in the absence and presence of 2.5 mM ADP-Mg2+, respectively | Archaeoglobus fulgidus | |
Mg2+ | required, bound to ATP | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | Archaeoglobus fulgidus | - |
ADP + phosphate + Cu+[side 2] | - |
? | |
ATP + H2O + Cu+[side 1] | Archaeoglobus fulgidus | the enzyme drives the efflux of Cu+ from the cell cytoplasm | ADP + phosphate + Cu+[side 2] | - |
? | |
additional information | Archaeoglobus fulgidus | in absence of other ligands, CopZ transfers Cu+ to the wild type enzyme CopA | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Archaeoglobus fulgidus | O29777 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | - |
Archaeoglobus fulgidus | ADP + phosphate + Cu+[side 2] | - |
? | |
ATP + H2O + Cu+[side 1] | the enzyme drives the efflux of Cu+ from the cell cytoplasm | Archaeoglobus fulgidus | ADP + phosphate + Cu+[side 2] | - |
? | |
ATP + H2O + Cu+[side 1] | mechanism of Cu+ transfer | Archaeoglobus fulgidus | ADP + phosphate + Cu+[side 2] | - |
? | |
additional information | in absence of other ligands, CopZ transfers Cu+ to the wild type enzyme CopA | Archaeoglobus fulgidus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CopA | - |
Archaeoglobus fulgidus |
Cu+ transport ATPase | - |
Archaeoglobus fulgidus |