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Literature summary for 7.2.2.8 extracted from

  • Völlmecke, C.; Drees, S.L.; Reimann, J.; Albers, S.V.; Lübben, M.
    The ATPases CopA and CopB both contribute to copper resistance of the thermoacidophilic archaeon Sulfolobus solfataricus (2012), Microbiology, 158, 1622-1633.
    View publication on PubMed
Search for more literature for 7.2.2.8

Cloned(Commentary)

Cloned (Comment) Organism
gene copA, phylogenetic analysis and sequence comparisons, recombinant expression of Sulfolobus solfataricus CopA in Escherichia coli strain DC194, deficient in the PIB-type copper export ATPase copA, yielding poor or partial complementation Saccharolobus solfataricus
gene copB, phylogenetic analysis and sequence comparisons, recombinant expression of Sulfolobus solfataricus CopB in Escherichia coli strain DC194, deficient in the PIB-type copper export ATPase copA, yielding poor or partial complementation Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information disruption of gene copA via allelic replacement does not affect the sensitivity of Sulfolobus solfataricus to reactive oxygen species Saccharolobus solfataricus
additional information disruption of gene copB via allelic replacement does not affect the sensitivity of Sulfolobus solfataricus to reactive oxygen species Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane an integral membrane protein of cytoplasmic membranes Saccharolobus solfataricus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu+[side 1] Saccharolobus solfataricus
-
 ADP + phosphate + Cu+[side 2]
-
 ?
ATP + H2O + Cu+[side 1] Saccharolobus solfataricus PBL2025
-
 ADP + phosphate + Cu+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
 gene copB
-
Saccharolobus solfataricus B2CQV8 gene copA
-
Saccharolobus solfataricus PBL2025
-
 gene copB
-
Saccharolobus solfataricus PBL2025 B2CQV8 gene copA
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu+[side 1]
-
 Saccharolobus solfataricus ADP + phosphate + Cu+[side 2]
-
 ?
ATP + H2O + Cu+[side 1]
-
 Saccharolobus solfataricus PBL2025 ADP + phosphate + Cu+[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
CopA
-
 Saccharolobus solfataricus
CopB
-
 Saccharolobus solfataricus

Expression

Organism Comment Expression
Saccharolobus solfataricus an increase in the external copper concentration induces the enzyme up

General Information

General Information Comment Organism
evolution the enzyme belongs to the PIB-ATPase family, phylogenetic analysis and tree Saccharolobus solfataricus
physiological function CopA is an effective copper pump at low and high copper concentrations. The enzyme is not a strict prerequisite to the biosynthesis of the copper protein cytochrome oxidase. CopA and CopB act as resistance factors to copper ions at overlapping concentrations, both ATPases are involved in resistance to silver Saccharolobus solfataricus
physiological function CopB is a low-affinity copper export ATPase, which is only relevant if the media copper concentration is exceedingly high. The enzyme is not a strict prerequisite to the biosynthesis of the copper protein cytochrome oxidase. CopA and CopB act as resistance factors to copper ions at overlapping concentrations, both ATPases are involved in resistance to silver Saccharolobus solfataricus