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Literature summary for 7.2.2.8 extracted from

  • Tsuda, T.; Toyoshima, C.
    Nucleotide recognition by CopA, a Cu+-transporting P-type ATPase (2009), EMBO J., 28, 1782-1791.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
with bound adenosine 5'-[beta, gamma-methylene]triphosphate or ADP and Mg2+, vapor diffusion method, using 5% (w/v) PEG 6000, 1.25-1.5M NaCl and 100 mM MES, pH 5.5-5.75, at 10°C Archaeoglobus fulgidus

Protein Variants

Protein Variants Comment Organism
H462Q the mutation reduces the affinity for adenosine 5'-[beta, gamma-methylene]triphosphate about 20fold Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu+[side 1] Archaeoglobus fulgidus
-
ADP + phosphate + Cu+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O29777
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose 6 column chromatography and Superdex 75 gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu+[side 1]
-
Archaeoglobus fulgidus ADP + phosphate + Cu+[side 2]
-
?

Synonyms

Synonyms Comment Organism
CopA
-
Archaeoglobus fulgidus
Cu+-transporting P-type ATPase
-
Archaeoglobus fulgidus