Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Archaeoglobus fulgidus |
Crystallization (Comment) | Organism |
---|---|
with bound adenosine 5'-[beta, gamma-methylene]triphosphate or ADP and Mg2+, vapor diffusion method, using 5% (w/v) PEG 6000, 1.25-1.5M NaCl and 100 mM MES, pH 5.5-5.75, at 10°C | Archaeoglobus fulgidus |
Protein Variants | Comment | Organism |
---|---|---|
H462Q | the mutation reduces the affinity for adenosine 5'-[beta, gamma-methylene]triphosphate about 20fold | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | Archaeoglobus fulgidus | - |
ADP + phosphate + Cu+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O29777 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni Sepharose 6 column chromatography and Superdex 75 gel filtration | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | - |
Archaeoglobus fulgidus | ADP + phosphate + Cu+[side 2] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CopA | - |
Archaeoglobus fulgidus |
Cu+-transporting P-type ATPase | - |
Archaeoglobus fulgidus |