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Literature summary for 7.2.2.8 extracted from

  • Chintalapati, S.; Al Kurdi, R.; van Scheltinga, A.C.; Kuehlbrandt, W.
    Membrane structure of CtrA3, a copper-transporting P-type-ATPase from Aquifex aeolicus (2008), J. Mol. Biol., 378, 581-595.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
L-cysteine isoform CtrA2 shows highest activity at 20 mM L-cysteine, isoform CtrA3 shows highest activity at 15 mM L-cysteine Aquifex aeolicus
additional information isoform CtrA2 is activated without added lipid, whereas the activity of isoform CtrA3 depended on the addition of extraneous lipid. Escherichia coli polar lipids, soybean phosphatidyl choline, synthetic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and a total extract of Aquifex aeolicus lipids all have roughly the same effect Aquifex aeolicus
NaCl highest activity at about 400 mM NaCl Aquifex aeolicus

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Aquifex aeolicus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Aquifex aeolicus

Localization

Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

Metals/Ions Comment Organism Structure
Ag+ isoform CtrA2 and CtrA3 are activated by Ag+ (with Ag+ being twice as effective as Cu+ in case of isoform CtrA2) Aquifex aeolicus
Cu+ isoform CtrA2 is activated by Cu+ Aquifex aeolicus
Cu2+ isoform CtrA3 is activated by Cu2+ Aquifex aeolicus
additional information not activated by Zn2+, Ni2+, Mg2+ or Co2+ Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
x * 78000, isoform CtrA3, SDS-PAGE Aquifex aeolicus
80000
-
x * 80000, isoform CtrA2, SDS-PAGE Aquifex aeolicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cu+[side 1] Aquifex aeolicus
-
ADP + phosphate + Cu+[side 2]
-
?

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-NTA column chromatography Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cu+[side 1]
-
Aquifex aeolicus ADP + phosphate + Cu+[side 2]
-
?

Subunits

Subunits Comment Organism
? x * 78000, isoform CtrA3, SDS-PAGE Aquifex aeolicus
? x * 80000, isoform CtrA2, SDS-PAGE Aquifex aeolicus

Synonyms

Synonyms Comment Organism
copper-transporting P-type-ATPase
-
Aquifex aeolicus
CtrA2 isoform Aquifex aeolicus
CtrA3 isoform Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aquifex aeolicus

General Information

General Information Comment Organism
physiological function isoform CtrA2 presumably transports reduced Cu+, while isoform CtrA3 presumably transports the oxidized Cu+ Aquifex aeolicus