Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-cysteine | isoform CtrA2 shows highest activity at 20 mM L-cysteine, isoform CtrA3 shows highest activity at 15 mM L-cysteine | Aquifex aeolicus | |
additional information | isoform CtrA2 is activated without added lipid, whereas the activity of isoform CtrA3 depended on the addition of extraneous lipid. Escherichia coli polar lipids, soybean phosphatidyl choline, synthetic 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and a total extract of Aquifex aeolicus lipids all have roughly the same effect | Aquifex aeolicus | |
NaCl | highest activity at about 400 mM NaCl | Aquifex aeolicus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
- |
Aquifex aeolicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | isoform CtrA2 and CtrA3 are activated by Ag+ (with Ag+ being twice as effective as Cu+ in case of isoform CtrA2) | Aquifex aeolicus | |
Cu+ | isoform CtrA2 is activated by Cu+ | Aquifex aeolicus | |
Cu2+ | isoform CtrA3 is activated by Cu2+ | Aquifex aeolicus | |
additional information | not activated by Zn2+, Ni2+, Mg2+ or Co2+ | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | - |
x * 78000, isoform CtrA3, SDS-PAGE | Aquifex aeolicus |
80000 | - |
x * 80000, isoform CtrA2, SDS-PAGE | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | Aquifex aeolicus | - |
ADP + phosphate + Cu+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | - |
Aquifex aeolicus | ADP + phosphate + Cu+[side 2] | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 78000, isoform CtrA3, SDS-PAGE | Aquifex aeolicus |
? | x * 80000, isoform CtrA2, SDS-PAGE | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
copper-transporting P-type-ATPase | - |
Aquifex aeolicus |
CtrA2 | isoform | Aquifex aeolicus |
CtrA3 | isoform | Aquifex aeolicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Aquifex aeolicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Aquifex aeolicus |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform CtrA2 presumably transports reduced Cu+, while isoform CtrA3 presumably transports the oxidized Cu+ | Aquifex aeolicus |