Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
C12E8 | - |
Thermotoga maritima | |
additional information | ATPase activity os almost completely suppressed by incubating CopA (1 mg/ml or 0.00633 mM) for 1 h in a solution containing 1 mM MgCl2, 0.1 mM AlCl3, 2 mM potassium fluoride in the presence of either 0.02 mM Cu+,1 mM ADP, and 0.02mM Cu+, or 0.5 mM bathocuproine disulfonate | Thermotoga maritima | |
N,N-Dimethyldodecylamine-N-oxide | - |
Thermotoga maritima | |
n-octyl beta-D-glucopyranoside | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0031 | - |
Cu+[side 1] | at pH 6.0 and 60°C | Thermotoga maritima |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Thermotoga maritima | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu+ | 100% activity at 0.02 mM Cu+ | Thermotoga maritima | |
Mg2+ | 100% activity at 1 mM Mg2+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83300 | - |
x * 83300, SELDI-TOF mass spectrometry | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | Thermotoga maritima | - |
ADP + phosphate + Cu+[side 2] | - |
? | |
additional information | Thermotoga maritima | the enzyme also shows Ag+-dependent ATPase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | Q9WYF3 | - |
- |
Purification (Comment) | Organism |
---|---|
Co2+ metal affinity resin column chromatography, and gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + Cu+[side 1] | - |
Thermotoga maritima | ADP + phosphate + Cu+[side 2] | - |
? | |
additional information | the enzyme also shows Ag+-dependent ATPase activity | Thermotoga maritima | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 83300, SELDI-TOF mass spectrometry | Thermotoga maritima |
? | x * 83400, calculated from amino acid sequence | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
CopA | - |
Thermotoga maritima |
Cu+-ATPase | - |
Thermotoga maritima |
TM0317 | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
Cu+-ATPase activity reaches the maximum at 60°C and is 4.4% of the maximum at 30°C | Thermotoga maritima |