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Literature summary for 7.2.2.3 extracted from
- Leishmania amazonensis: characterization of an ouabain-insensitive Na+-ATPase activity (2008), Exp. Parasitol., 118, 165-171.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Na+ | - |
Leishmania amazonensis |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Furosemide | - |
Leishmania amazonensis | |
| sodium orthovanadate | - |
Leishmania amazonensis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Leishmania amazonensis | 5886 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania amazonensis | - |
MHOM/BR/75/Josefa strain | - |
| Leishmania amazonensis MHOM/BR/75/Josefa | - |
MHOM/BR/75/Josefa strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| plasma membranes are isolated from the promastigotes of Leishmania amazonensis | Leishmania amazonensis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00325 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control | Leishmania amazonensis |
| 0.00336 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control | Leishmania amazonensis |
| 0.00734 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control, 10 mM MgCl2 | Leishmania amazonensis |
| 0.00741 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control, 10 mM MgCl2 | Leishmania amazonensis |
| 0.00808 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control, 10 mM MgCl2, 120 mM NaCl | Leishmania amazonensis |
| 0.00813 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control, 10 mM MgCl2, 120 mM NaCl | Leishmania amazonensis |
| 0.00818 | - |
ATPase activity in homogenate, hydrolysis of ATP, with 1 mM ouabain, control, 10 mM MgCl2, 120 mM NaCl, 30 mM KCl | Leishmania amazonensis |
| 0.00907 | - |
ATPase activity in homogenate, hydrolysis of ATP, without ouabain, control, 10 mM MgCl2, 120 mM NaCl, 30 mM KCl | Leishmania amazonensis |
| 0.0103 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, control | Leishmania amazonensis |
| 0.0272 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, 10 mM MgCl2 | Leishmania amazonensis |
| 0.0286 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, 10 mM MgCl2, 120 mM NaCl, 30 mM KCl | Leishmania amazonensis |
| 0.0294 | - |
ATPase activity in plasma membranes in the presence of 1 mM ouabain, hydrolysis of ATP, 10 mM MgCl2, 120 mM NaCl | Leishmania amazonensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Na+-ATPase | - |
Leishmania amazonensis |
| Na+-exporting ATPase | - |
Leishmania amazonensis |
| P-type Na+-ATPase | - |
Leishmania amazonensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATPase activity assay | Leishmania amazonensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
ATPase activity assay | Leishmania amazonensis |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
- |
Leishmania amazonensis | sodium orthovanadate | |
| 0.27 | - |
- |
Leishmania amazonensis | Furosemide | |
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Release 2023.1 (January 2023)
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