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Literature summary for 7.2.2.3 extracted from
- Molecular cloning and characterization of ouabain-insensitive Na+-ATPase in the parasitic protist, Trypanosoma cruzi (2006), Biochim. Biophys. Acta, 1758, 738-746.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| K+ | - |
Trypanosoma cruzi |  |
| Na+ | - |
Trypanosoma cruzi | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into tht pCR II-TOPO vector, into the mammalian expression vector pEGFP-N1 for transfection of FreeStyle 293-F cells, and into the trypanosomatid shuttle vector pTREX for transformation of Trypanosoma cruzi epimastigotes | Trypanosoma cruzi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| orthovanadate | - |
Trypanosoma cruzi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Trypanosoma cruzi | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
calculated molecular mass, determined by SDS-PAGE and Western-blotting | Trypanosoma cruzi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Na+/in | Trypanosoma cruzi | - |
ADP + phosphate + Na+/out | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | Q76DT8 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Na+/in | - |
Trypanosoma cruzi | ADP + phosphate + Na+/out | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ENA | - |
Trypanosoma cruzi |
| Na+-ATPase | - |
Trypanosoma cruzi |
| Na+-exporting ATPase | - |
Trypanosoma cruzi |
| TcENA | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
ATPase activity assay | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
ATPase activity assay | Trypanosoma cruzi |
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Release 2023.1 (January 2023)
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