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Literature summary for 7.2.2.3 extracted from

  • Becker, S.; Schneider, H.; Schneider-Bovis, G.
    The highly conserved extracellular peptide, DSYG(893-896), is a critical structure for sodium pump function (2004), Eur. J. Biochem., 271, 3821-3831.
    View publication on PubMed
Search for more literature for 7.2.2.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sacharomyces cerevisiae Ovis aries

Protein Variants

Protein Variants Comment Organism
D893A mutant with no significant Na+/K+ ATPase activity Ovis aries
D893E mutant with no significant Na+/K+ ATPase activity Ovis aries
D893R mutant with no significant Na+/K+ ATPase activity Ovis aries
G896I mutant with no significant Na+/K+ ATPase activity Ovis aries
G896R mutant with no significant Na+/K+ ATPase activity Ovis aries
S894D mutant with similar Na+/K+ ATPase activity as the wild-type enzyme Ovis aries
S894I mutant with similar Na+/K+ ATPase activity as the wild-type enzyme Ovis aries
Y895C mutant with similar Na+/K+ ATPase activity as the wild-type enzyme Ovis aries
Y895F mutant with similar Na+/K+ ATPase activity as the wild-type enzyme Ovis aries
Y895W mutant with significantly reduced Na+/K+ ATPase activity Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information EC50 of 0.00072 mM, 0.00107 mM and 0.0008 mM for Y895C, Y895F and Y895W mutants, respectively, with ATP as substrate. EC50 of 1.44 mM, 1.36 mM and 1.87 mM for Y895C, Y895F and Y895W mutants, respectively, with Na+ as substrate. EC50 of 3.74 mM, 1.75 mM and 2.78 mM for Y895C, Y895F and Y895W mutants, respectively, with K+ as substrate Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0076
-
 Y895W mutant, 37ºC Ovis aries
0.01316
-
 Y895C mutant, 37ºC Ovis aries
0.0133
-
 Y895F mutant, 37ºC Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Na+/in
-
 Ovis aries ADP + phosphate + Na+/out
-
 ?

Synonyms

Synonyms Comment Organism
EC 3.6.3.9 related Ovis aries

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 62%, 67.6% and 51.4% residual activity for wild-type, Y895F and YY895W mutant enzymes. Only 36.5% residual activity for Y895C mutant. The aromatic group contributes to thermal stability Ovis aries