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Literature summary for 7.2.2.3 extracted from
- Purification and characterization of the catalytic moiety of vacuolar-type Na+-ATPase from Enterococcus hirae (1994), J. Biochem., 116, 1302-1308.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-Chloro-4-nitrobenzo-2-oxa-1,3-diazole | - |
Enterococcus hirae |  |
| Dicyclohexylcarbodiimide | - |
Enterococcus hirae | |
| N-ethylmaleimide | - |
Enterococcus hirae | |
| nitrate | - |
Enterococcus hirae | |
| Sodium azide | - |
Enterococcus hirae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | - |
Enterococcus hirae | |
| Na+ | - |
Enterococcus hirae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 52000 | - |
3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 69000 | - |
3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
| 400000 | - |
gel filtration | Enterococcus hirae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Na+/in | Enterococcus hirae | - |
ADP + phosphate + Na+/out | - |
r | |
| CTP + H2O | Enterococcus hirae | - |
? | - |
? | |
| GTP + H2O | Enterococcus hirae | - |
? | - |
? | |
| UTP + H2O | Enterococcus hirae | - |
? | - |
? | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Glycerol | - |
Enterococcus hirae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus hirae | - |
strain ATCC 9790 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus hirae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.73 | - |
- |
Enterococcus hirae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + Na+/in | - |
Enterococcus hirae | ADP + phosphate + Na+/out | - |
r | |
| CTP + H2O | - |
Enterococcus hirae | ? | - |
? | |
| GTP + H2O | - |
Enterococcus hirae | ? | - |
? | |
| UTP + H2O | - |
Enterococcus hirae | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| oligomer | 3 * 69000 + 3 * 52000 + 1 * 29000, SDS-PAGE | Enterococcus hirae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Enterococcus hirae |
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