Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.2.21 extracted from

  • Adle, D.J.; Lee, J.
    Expressional control of a cadmium-transporting P1B-type ATPase by a metal sensing degradation signal (2008), J. Biol. Chem., 283, 31460-31468.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.21

Activating Compound

Activating Compound Comment Organism Structure
Ca2+ the inducing effect of cadmium on Pca1 expression is independent of epitope or its location Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C298A/C300A site-directed mutation of the CXC motif abolishes copper resistance but not cadmium resistance Saccharomyces cerevisiae
C311A/C312A site-directed mutation of the CC motif abolishes cadmium resistance yet retains the ability to confer copper resistance Saccharomyces cerevisiae
additional information deletion of the N-terminal extension domain results in constitutive expression of Pca1 at the cell surface even in the absence of cadmium. Pca1 is not stabilized in a yeast strain defective in endocytosis, GFP-Pca1 does not accumulate in the vacuole of the DELTApep4 strain Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane Pca1 contains a cytosolic cysteine-rich N-terminal extension of nearly 400 residue Saccharomyces cerevisiae 5886
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cd2+/in Saccharomyces cerevisiae Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Cd2+/in Saccharomyces cerevisiae BY4741 Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane ADP + phosphate + Cd2+/out
-
 ?
additional information Saccharomyces cerevisiae mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway ?
-
 ?
additional information Saccharomyces cerevisiae BY4741 mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 BY4741 haploid strain
-
Saccharomyces cerevisiae BY4741
-
 BY4741 haploid strain
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd2+/in Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane Saccharomyces cerevisiae ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Cd2+/in the N-terminus of Pca1 contains a metal-responding degradation signal encompassing amino acids 250-350. Seven cysteine residues within the Pca1 regulatory domain are required for metal sensing Saccharomyces cerevisiae ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Cd2+/in Pca1 plays a critical role in cadmium resistance by extruding intracellular cadmium. Yeast cells rapidly enhance expression of Pca1 by a post-transcriptional mechanism. In the absence of cadmium, Pca1 is targeted for degradation before reaching the plasmamembrane Saccharomyces cerevisiae BY4741 ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Cd2+/in the N-terminus of Pca1 contains a metal-responding degradation signal encompassing amino acids 250-350. Seven cysteine residues within the Pca1 regulatory domain are required for metal sensing Saccharomyces cerevisiae BY4741 ADP + phosphate + Cd2+/out
-
 ?
additional information mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway Saccharomyces cerevisiae ?
-
 ?
additional information mechanism for substrate-mediated control of P1B-type ATPase expression, overview. Dependence of Pca1 degradation through the vacuolar/endosomal pathway Saccharomyces cerevisiae BY4741 ?
-
 ?

Synonyms

Synonyms Comment Organism
cadmium-transporting P1B-type ATPase
-
 Saccharomyces cerevisiae
Pca1,aP1B-type ATPase
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae