Literature summary for 7.2.2.2 extracted from

Sasser, T.L.; Lawrence, G.; Karunakaran, S.; Brown, C.; Fratti, R.A.
The yeast ATP-binding cassette (ABC) transporter Ycf1p enhances the recruitment of the soluble SNARE Vam7p to vacuoles for efficient membrane fusion (2013), J. Biol. Chem., 288, 18300-18310.

Search for more literature for 7.2.2.2

Protein Variants

Protein Variants	Comment	Organism
K669M	enzyme Ycf1p containing a mutation of the conserved Lys669 to Met in the Walker A box of the first nucleotide-binding domain (Ycf1pK669M) is unable to complement the fusion defect of DELTAycf1 vacuoles	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
vacuole	-	Saccharomyces cerevisiae	5773	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Saccharomyces cerevisiae	enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P39109	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme Ycf1p regulates phosphatidylinositol 3-phosphate accumulation at vertex microdomains	Saccharomyces cerevisiae	?	-	?

Synonyms

Synonyms	Comment	Organism
Ycf1p	-	Saccharomyces cerevisiae

General Information

General Information	Comment	Organism
evolution	the enzyme belongs to the ATP-binding cassette class C (ABCC) transporter family	Saccharomyces cerevisiae
malfunction	deletion of YCF1 attenuates in vitro vacuole fusion by up to 40% relative to wild-type vacuoles, plasmid-expressed wild-type Ycf1p rescues the deletion phenotype. Ycf1p containing a mutation of the conserved Lys669 to Met in the Walker A box of the first nucleotide-binding domain (Ycf1pK669M) is unable to complement the fusion defect of DELTAycf1 vacuoles. Also, deleting YCF1 causes a striking decrease in vacuolar levels of the soluble SNARE Vam7p, whereas total cellular levels are not altered. The attenuated fusion of DELTAycf1 vacuoles is rescued by the addition of recombinant Vam7p to in vitro experiments, exogenous Vam7p restores Ca2+ efflux in DELTAycf1 vacuoles. Phenotypes, overview	Saccharomyces cerevisiae
physiological function	the enzyme Ycf1p is a Cd2+ transporter, it regulates the transport of cadmium, mercury, and other toxins into the vacuole lumen to detoxify the cytoplasm. The ATPase activity of Ycf1p is required for its function in regulating fusion. The enzyme physically interacts with a wide array of proteins, including factors that regulate vacuole homeostasis. Role of Ycf1p and other ABCC transporters in the regulation of vacuole homotypic fusion, overview. Ycf1p contributes in the recruitment of Vam7p to the vacuole for efficient membrane fusion	Saccharomyces cerevisiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)