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Literature summary for 7.2.2.2 extracted from

  • Paumi, C.M.; Chuk, M.; Chevelev, I.; Stagljar, I.; Michaelis, S.
    Negative regulation of the yeast ABC transporter Ycf1p by phosphorylation within its N-terminal extension (2008), J. Biol. Chem., 283, 27079-27088.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.2

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is activated by phosphorylation in its ABC core domain at residues Ser908 and Thr911 Saccharomyces cerevisiae
Tus1p a guanine nucleotide exchange factor, stimulates the enzyme Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
construction of an integrated membrane yeast two-hybrid, iMYTH, screen and identification of two kinase genes, CKA1 and HAL5, deletion of which increases Ycf1p function Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information deletion of the two kinase genes CKA1 and HAL5 increases Ycf1p function Saccharomyces cerevisiae
S251A the site-directed mutation inhibits phosphorylation, the mutant exhibits increased resistance to cadmium in vivo and increased Ycf1p-dependent transport of [3H]estradiol-beta-17-glucuronide in vitro as compared with wild-type Ycf1p Saccharomyces cerevisiae
S251E the site-directed mutation mimics phosphorylation, activity is restored to the wild-type level for mutant Ycf1-S251E Saccharomyces cerevisiae
S908A/T911A the mutant shows a 50% diminished expression of Ycf1p compared to the wild-type enzyme Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information phosphorylation of Ser251 in the Ycf1p N-terminal extension negatively regulates activity Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.187
-
 estradiol-beta-17-glucuronide [side 1] mutant S251A Saccharomyces cerevisiae
0.394
-
 estradiol-beta-17-glucuronide [side 1] wild-type enzyme Saccharomyces cerevisiae
0.481
-
 estradiol-beta-17-glucuronide [side 1] mutant S251E Saccharomyces cerevisiae
0.548
-
 estradiol-beta-17-glucuronide [side 1] mutant S908A/T911A Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
 Saccharomyces cerevisiae 5774
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + Cd2+/in Saccharomyces cerevisiae the yeast ABC transporter belongs to the ABCC subfamily of ATP-binding cassette, ABC, transporters that rid cells of toxic endogenous and xenobiotic compounds, negative regulation of Ycf1p by phosphorylation within its N-terminal extension in addition to its ABC core domain and transports substrates in the form of glutathione conjugates ADP + phosphate + Cd2+/out
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is activated by phosphorylation in its ABC core domain at residues Ser908 and Thr911, while phosphorylation of Ser251 in the Ycf1p N-terminal extension negatively regulates activity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 measurement of growth inhibition by cadmium in wild-type and mutant strains, overview Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + Cd2+/in the yeast ABC transporter belongs to the ABCC subfamily of ATP-binding cassette, ABC, transporters that rid cells of toxic endogenous and xenobiotic compounds, negative regulation of Ycf1p by phosphorylation within its N-terminal extension in addition to its ABC core domain and transports substrates in the form of glutathione conjugates Saccharomyces cerevisiae ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + Cd2+/in Ycf1p-dependent transport of [3H]estradiol-beta-17-glucuronide in vitro Saccharomyces cerevisiae ADP + phosphate + Cd2+/out
-
 ?
ATP + H2O + estradiol-beta-17-glucuronide [side 1]
-
 Saccharomyces cerevisiae ADP + phosphate + estradiol-beta-17-glucuronide [side 2]
-
 ?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the ABCC subfamily of ABC transporters Saccharomyces cerevisiae
Ycf1p
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae