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Literature summary for 7.2.2.19 extracted from

  • Dubey, V.; Han, M.; Kopec, W.; Solovyov, I.A.; Abe, K.; Khandelia, H.
    K+ binding and proton redistribution in the E2P state of the H+, K+-ATPase (2018), Sci. Rep., 8, 12732 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.2.2.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in HEK-293S GnT1- cells Sus scrofa

Protein Variants

Protein Variants Comment Organism
E343A the mutant has no activity Sus scrofa
E343L the mutant has no activity Sus scrofa
E343Q the mutation demonstrates reduced activity Sus scrofa
E343V the mutant has no activity Sus scrofa
E795Q the mutant shows increased affinity for K+ compared to the wild type enzyme Sus scrofa
E820D the mutant remains active Sus scrofa
E820Q the mutant has K+-independent constitutive dephosphorylation activity and an increased preference for the E1 conformation Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
SCH28080 i.e., 2-methyl-8-(phenylmethoxy)imidazo[1,2-a]pyridine-3-acetonitrile Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 K+[side 2] mutant enzyme E795Q, at pH 7.0 and 37°C Sus scrofa
1.2
-
 K+[side 2] wild type enzyme, at pH 7.0 and 37°C Sus scrofa
5.5
-
 K+[side 2] mutant enzyme E343Q, at pH 7.0 and 37°C Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
plasma membrane
-
 Sus scrofa 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 2 mM used in assay conditions Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + H+[side 1] + K+[side 2] Sus scrofa
-
 ADP + phosphate + H+[side 2] + K+[side 1]
-
 ?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stomach
-
 Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+[side 1] + K+[side 2]
-
 Sus scrofa ADP + phosphate + H+[side 2] + K+[side 1]
-
 ?

Synonyms

Synonyms Comment Organism
H+, K+-ATPase
-
 Sus scrofa
HKA
-
 Sus scrofa