Cloned (Comment) | Organism |
---|---|
expression of non-gastric enzyme in Spodoptera frugiperda Sf9 cells via baculovirus transfection, co-expression with the Rattus norvegicus beta1 subunit of Na,K-ATPase | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oligomycin | increases the phosphorylation of the enzyme and the sensitivity of the phosphorylated intermediate to AD inhibiting the conversion of E1P to E2P | Homo sapiens | |
oubain | - |
Homo sapiens | |
SCH 28080 | inhibits the NH4+-stimulated activity | Homo sapiens | |
vanadate | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
ATP | pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ | Homo sapiens | |
0.4 | - |
ATP | pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ | Homo sapiens | |
1 | - |
K+ | pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ | Homo sapiens | |
1.2 | - |
ATP | pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ | Homo sapiens | |
2 | - |
K+ | pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ | Homo sapiens | |
20 | - |
K+ | pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | low activation by K+, but stimulates enzyme dephosphorylation | Homo sapiens | |
Mg2+ | required for ATPase activity | Homo sapiens | |
Na+ | activates, stimulates enzyme dephosphorylation | Homo sapiens | |
NH4+ | best activating cation, stimulates enzyme dephosphorylation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in + K+/out | Homo sapiens | - |
ADP + phosphate + H+/out + K+/in | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the steady-state phopshorylation level of the enzyme decreases with increasing pH, K+ and Na+ concentration, but nearly doubles in presence of oligomycin, Na+, K+, and NH4+ stimulate enzyme dephosphorylation | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant non-gastric enzyme partially from Spodoptera frugiperda Sf9 cells by membrane preparation | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
stomach | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.186 | - |
partially purified recombinant enzyme, in presence of 10 mM K+ | Homo sapiens |
0.611 | - |
partially purified recombinant enzyme, in presence of 100 mM Na+ | Homo sapiens |
0.685 | - |
partially purified recombinant enzyme, in presence of 10 mM NH4+ | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + H+/in + K+/out | - |
Homo sapiens | ADP + phosphate + H+/out + K+/in | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme belongs to the P-type ATPases | Homo sapiens |
non-gastric H,K-ATPase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0008 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ | Homo sapiens | vanadate | |
0.0013 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ | Homo sapiens | vanadate | |
0.0016 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ | Homo sapiens | vanadate | |
0.003 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+, or 10 mM K+, or 100 mM Na+ | Homo sapiens | oligomycin | |
0.02 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ | Homo sapiens | SCH 28080 | |
0.03 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ | Homo sapiens | SCH 28080 | |
0.06 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 100 mM Na+ | Homo sapiens | oubain | |
0.4 | - |
pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM NH4+ | Homo sapiens | oubain | |
4 | - |
above, pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ | Homo sapiens | SCH 28080 | |
8 | - |
above, pH 7.0, 37°C, recombinant enzyme, in presence of 10 mM K+ | Homo sapiens | oubain |