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Literature summary for 7.2.2.19 extracted from

  • Qiu Li, Y.; Swarts Herman, G.P.; Tonk Elisa, C.M.; Willems Peter, H.G.M.; Koenderink Jan, B.; De Pont Jan Joep, H.H.M.
    Conversion of the low affinity ouabain-binding site of non-gastric H,K-ATPase into a high affinity binding site by substitution of only five amino acids (2006), J. Biol. Chem., 281, 13533-13539.
    View publication on PubMed
Search for more literature for 7.2.2.19

Activating Compound

Activating Compound Comment Organism Structure
NH4+ stimulates ATPase activity of non-gastric H,K-ATPase-EGPLC by 40% as compared to wild-type Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
catalytic subunit of the enzyme, in which several amino acids from Na,K-ATPase are incorporated, expressed with the Na,K-ATPase beta1 subunit in Xenopus laevis oocytes, expression of non-gastric H,K-ATPase-EGPLC and the Na,K-ATPase beta1-subunit in Sf9 cells using the baculovirus expression system Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
A778P/C781T expression level rather similar to that of the recombinant enzyme, ability of binding 3(H)ouabain with high affinity Rattus norvegicus
A778P/C781T/Y786F/V788I/G790N/L791I/I795L/I798V/F802C expression level and 3(H)ouabain binding level rather similar to that of the recombinant enzyme Rattus norvegicus
I795L/I798V/F802C expression level rather similar to that of the recombinant enzyme, ability of binding 3(H)ouabain with high affinity Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G expression level rather similar to that of the recombinant enzyme, ability of binding 3(H)ouabain with high affinity Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C expression level rather similar, but 3(H)ouabain binding level significantly higher than that of the recombinant enzyme Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/C802F 3(H)ouabain binding level significantly lower than that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/E312D 3(H)ouabain binding level significantly lower than that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/G319S 3(H)ouabain binding level significantly lower than that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/L795I 3(H)ouabain binding level significantly lower than that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/P778A 3(H)ouabain binding level significantly lower than that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/T309Y 3(H)ouabain binding level not significantly different from that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/T781C 3(H)ouabain binding level not significantly different from that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/V314I 3(H)ouabain binding level not significantly different from that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/V798I 3(H)ouabain binding level not significantly different from that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C/W310V 3(H)ouabain binding level significantly higher that of mutant Y309T/V310W/D312E/I314V/S319G/A778P/C781T/I795L/I798V/F802C Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/Y786F/V788I/G790N/L791I expression level and 3(H)ouabain binding level rather similar to that of the recombinant enzyme Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/A778P/C781T/Y786F/V788I/G790N/L791I/I795L/I798V/F802C expression level rather similar, but 3(H)ouabain binding level significantly higher than that of the recombinant enzyme Rattus norvegicus
Y309T/V310W/D312E/I314V/S319G/Y786F/V788I/G790N/L791I/I795L/I798V/F802C expression level and 3(H)ouabain binding level rather similar to that of the recombinant enzyme Rattus norvegicus
Y786F/V788I/G790N/L791I expression level rather similar to that of the recombinant enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
oubain binding affinity of wild-type is 2000fold lower than that of mutated enzyme in NH4+-stimulated Sf9 cells Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + H+/in + K+/out
-
 Rattus norvegicus ADP + phosphate + H+/out + K+/in
-
 ?

Synonyms

Synonyms Comment Organism
non-gastric H,K-ATPase
-
 Rattus norvegicus