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Literature summary for 7.2.2.19 extracted from

  • Abe, K.; Kaya, S.; Taniguchi, K.; Hayashi, Y.; Imagawa, T.; Kikumoto, M.; Oiwa, K.; Sakaguchi, K.
    Evidence for a relationship between activity and the tetraprotomeric assembly of solubilized pig gastric H/K-ATPase (2005), J. Biochem., 138, 293-301.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no protective effect during C12E8-solubilization of gastric H/K-ATPase by choline Sus scrofa

Application

Application Comment Organism
additional information strong evidence that the catalytic unit of C12E8-solubilized gastric H/K-ATPase is a tetraprotomer Sus scrofa

Inhibitors

Inhibitors Comment Organism Structure
Fluorescein 5'-isothiocyanate fluorescein 5'-isothiocyanate modified enzyme has 0.5-1.5% residual ATPase activity compared to the unmodified enzyme Sus scrofa
SCH28080
-
Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Sus scrofa 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stabilizes both oligomeric assembly and enzyme activity in C12E8-solubilized gastric H/K-ATPase, 20-40 mM protects Sus scrofa
additional information no protective effect during C12E8-solubilization of gastric H/K-ATPase by Na+ and Li+ Sus scrofa
Rb+ protects gastric H/K-ATPase during C12E8-solubilization Sus scrofa

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by SDS-PAGE Sus scrofa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3
-
purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C Sus scrofa
7.5
-
purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C Sus scrofa

Storage Stability

Storage Stability Organism
-80°C, EGTA/Tris buffer, pH 7.4 Sus scrofa

Synonyms

Synonyms Comment Organism
gastric H/K-ATPase
-
Sus scrofa