Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no protective effect during C12E8-solubilization of gastric H/K-ATPase by choline | Sus scrofa |
Application | Comment | Organism |
---|---|---|
additional information | strong evidence that the catalytic unit of C12E8-solubilized gastric H/K-ATPase is a tetraprotomer | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Fluorescein 5'-isothiocyanate | fluorescein 5'-isothiocyanate modified enzyme has 0.5-1.5% residual ATPase activity compared to the unmodified enzyme | Sus scrofa | |
SCH28080 | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Sus scrofa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | stabilizes both oligomeric assembly and enzyme activity in C12E8-solubilized gastric H/K-ATPase, 20-40 mM protects | Sus scrofa | |
additional information | no protective effect during C12E8-solubilization of gastric H/K-ATPase by Na+ and Li+ | Sus scrofa | |
Rb+ | protects gastric H/K-ATPase during C12E8-solubilization | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by SDS-PAGE | Sus scrofa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.3 | - |
purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C | Sus scrofa |
7.5 | - |
purified enzyme, 40 mM HEPES/Tris, 10 mM KCl, 2 mM MgCl2, 25 mM sucrose, 0.1 mM EGTA/Tris, 2 mM ATP/Tris pH 7.0, at 37°C | Sus scrofa |
Storage Stability | Organism |
---|---|
-80°C, EGTA/Tris buffer, pH 7.4 | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
gastric H/K-ATPase | - |
Sus scrofa |