Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.2.2.19 extracted from

  • Chatterjee, P.K.; Das, P.K.
    Characterization of Syrian hamster gastric mucosal H+,K+-ATPase (1995), Mol. Cell. Biochem., 148, 95-103.
    View publication on PubMed
Search for more literature for 7.2.2.19

Inhibitors

Inhibitors Comment Organism Structure
amiloride
-
 Mesocricetus auratus
Ca2+ 2 mM, complete inhibition Mesocricetus auratus
F-
-
 Mesocricetus auratus
Mn2+ in presence of Mg2+ Mesocricetus auratus
N,N'-dicyclohexylcarbodiimide
-
 Mesocricetus auratus
Na+ above 100 mM, 60-70% inhibition Mesocricetus auratus
Nbd-Cl
-
 Mesocricetus auratus
oligomycin
-
 Mesocricetus auratus
Omeprazole
-
 Mesocricetus auratus
SCH28080
-
 Mesocricetus auratus
vanadate
-
 Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.34
-
 K+
-
 Mesocricetus auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mesocricetus auratus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ activates, Km: 1.25 mM, monovalent cations stimulate in the order of decreasing efficacy: Tl+, K+, Rb+, NH4+, Cs+ Mesocricetus auratus
K+ maximum activation at 5-10 mM, Km: 0.34 mM, monovalent cations stimulate in the order of decreasing efficacy: Tl+, K+, Rb+, NH4+, Cs+ Mesocricetus auratus
Mg2+ activates Mesocricetus auratus
Mn2+ 77% of the activation with Mg2+, in absence of Mg2+ Mesocricetus auratus
NH4+ activates, Km: 3.64 mM, monovalent cations stimulate in the order of decreasing efficacy: Tl+, K+, Rb+, NH4+, Cs+ Mesocricetus auratus
Rb+ activates, Km: 0.43 mM, monovalent cations stimulate in the order of decreasing efficacy: Tl+, K+, Rb+, NH4+, Cs+ Mesocricetus auratus
Tl+ activates, Km: 0.03 mM, monovalent cations stimulate in the order of decreasing efficacy: Tl+, K+, Rb+, NH4+, Cs+ Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
 i.e. Syrian hamster
-

Source Tissue

Source Tissue Comment Organism Textmining
stomach gastric mucosa Mesocricetus auratus
-

Storage Stability

Storage Stability Organism
-80°C, in glycerol containing buffer, stable for more than 6 months Mesocricetus auratus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-deoxyATP + H2O + H+/in + K+/out 70% of the activity with ATP Mesocricetus auratus 2'-deoxyADP + phosphate + H+/out + K+/in
-
 ?
ADP + H2O + H+/in + K+/out 14% of the activity with ATP Mesocricetus auratus AMP + phosphate + H+/out + K+/in
-
 ?
ATP + H2O + H+/in + K+/out
-
 Mesocricetus auratus ADP + phosphate + H+/out + K+/in
-
 ?
CTP + H2O + H+/in + K+/out 20% of the activity with ATP Mesocricetus auratus CDP + phosphate + H+/out + K+/in
-
 ?
GTP + H2O + H+/in + K+/out 6% of the activity with ATP Mesocricetus auratus GDP + phosphate + H+/out + K+/in
-
 ?
ITP + H2O + H+/in + K+/out 5% of the activity with ATP Mesocricetus auratus IDP + phosphate + H+/out + K+/in
-
 ?
UTP + H2O + H+/in + K+/out 40% of the activity with ATP Mesocricetus auratus UDP + phosphate + H+/out + K+/in
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Mesocricetus auratus